广西香港海鸥菌分离菌株脉冲场凝胶电泳分子分型研究  

Molecular typing of Laribacter hongkonggensis isolated in Guangxi province by pulsed-field gel electrophoresis

作　　者：孙贵娟[1] 黄彦[1] 唐振柱[1] 卢桂宁[1] 苏伟东[1] 甘永新[1] 

机构地区：[1]广西壮族自治区疾病预防控制中心,广西南宁530028

出　　处：《中国食品卫生杂志》2011年第6期494-496,共3页Chinese Journal of Food Hygiene

摘　　要：目的探讨香港海鸥菌分子分型方法,了解广西水产品监测所分离的香港海鸥菌的相关性。方法以NotⅠ限制性内切酶对2005年分离的香港海鸥菌酶切后进行脉冲电泳,用BioNumerics 5.1聚类分析获得电泳图谱。结果 7株香港海鸥菌分为6个分子型,其中从南宁分离的与从河池分离的2株香港海鸥菌高度同源,相似度达100%。结论 PFGE可应用于香港海鸥菌分子分型,有助于发现香港海鸥菌流行规律和传播途径,水鸟可能是香港海鸥菌传播环节的一种重要媒介。Objective To study the molecular typing of Laribacter hongkonggensis by pulsed-field gel electrophoresis（PFGE）,and to find out the relationship of 7 strains of L.hongkonggensis isolated from fishery products in Guangxi on 2005.Methods Total DNA of each isolate was digested with restriction enzyme Not I,and the DNA fingerprints were obtained by running PFGE.These fingerprints of different stains were compared and their relationship was investigated by BioNumerics 5.1 clustering.Results Seven strains of L.hongkonggensis were attributed to six PFGE pattern combinations.One isolate from Nanning and another one from Hechi were homologous with 100% similarity.Conclusion PFGE is an effective way for molecular typing L.hongkonggensis and is helpful in discovering the epidemiology and transmission of this bacteria.Waterfowl might be an important medium in the spreading of L.hongkonggensis.

关 键 词：香港海鸥菌 脉冲场凝胶电泳 分子分型 食源性病原菌 媒介 

分 类 号：R378[医药卫生—病原生物学]