马蔺子素化学成分的研究  

Separation of chemical constituents from irisquinone

作　　者：牛剑钊[1] 刘怀林[2] 张启明[1] 

机构地区：[1]中国食品药品检定研究院,北京100050 [2]山东新华制药股份有限公司,淄博255005

出　　处：《中国新药杂志》2011年第22期2251-2253,共3页Chinese Journal of New Drugs

摘　　要：目的:使用制备液相分离提纯马蔺子素中有效成分,并对其结构进行研究。方法:采用AgelaVenusil XBP C18半制备柱(250 mm×10 mm,10μm)1,00;流动相为甲醇-乙醚(75∶25)-水(70∶30);流速10mL.min-1;检测波长270 nm。结果:所得化合物结构经元素吸收、红外、质谱和核磁共振法确证,经分析型HPLC检查,纯度达99.0%以上。结论:分离得到两个主要成分,确证为马蔺子甲素和6-甲氧基-2-十七烷基-14,-苯醌。Objective： To separate chemical constituents from irisquinone by preparative liquid chromatography and study its structure.Methods： The separation was performed on Agela Venusil XBP C18（250 mm×10 mm,10 μm） column with organic phases [a mixture of methanol and ethylether（75∶25）]-water（70∶30） as mobile phase.The flow rate was 10 mL·min-1,and 270 nm was used as detective wavelength.Results： Their chemical structures were identified by elementary analysis,IR,1H-NMR and 13C-NMR.The purity of chemical constituents was over 99.0% by HPLC.Conclusion： Two chemical constituents have been isolated and confirmed.

关 键 词：马蔺子素 分离 制备 波谱分析 

分 类 号：R927.1[医药卫生—药学]