4-1BBL/4-1BB信号对系统性红斑狼疮患者外周血淋巴细胞活化的影响  

The effect of costimulatory signal 4-1 BBL/4-1 BB on the activation of peripheral blood lymphocytes with systemic lupus erythematosus

作　　者：高元元[1] 吴俊英[1,2] 李柏青[2] 王慧[1] 梁友宝[2] 梁华[2] 

机构地区：[1]蚌埠医学院临床检验诊断学实验中心,安徽蚌埠233030 [2]蚌埠医学院免疫学教研室,安徽蚌埠233030

出　　处：《蚌埠医学院学报》2011年第11期1177-1181,共5页Journal of Bengbu Medical College

摘　　要：目的:探讨4-1BBL/4-1BB信号对系统性红斑狼疮(SLE)患者外周血淋巴细胞活化及共刺激分子表达的影响。方法:用CD3单抗刺激SLE患者外周血单个核细胞,并用抗4-1BBL单抗阻断4-1BBL/4-1BB共刺激信号,流式细胞术检测阻断前后T、B细胞上4-1BB、CD40L、4-1BBL、CD40以及CD69表达水平。结果:SLE患者T细胞上CD69表达明显高于正常对照组(P<0.01);SLE患者外周血T细胞上4-1BB、CD40L和B细胞上4-1BBL、CD40表达明显高于正常对照组(P<0.01),活化后T细胞上共刺激分子表达升高更加明显(P<0.01);抗4-1BBL单抗阻断后4-1BB、CD40L表达明显下降(P<0.01),B细胞上CD40表达下降,与SLE未活化组和活化组比较差异有统计学意义(P<0.05)。结论:SLE患者外周血T、B细胞活化水平升高,4-1BBL/4-1BB信号对其活化状态维持可以起重要作用。Objective：To investigate the effect of costimulatory signal 4-1BBL/4-1 BB in the activation of the T lymphocytes and the expression of costimulatory molecule of patients with（SLE）.Methods：The peripheral blood mononuclear cells（PBMCs） were stimulated with anti-CD3 and the costimulatory signal of 4-1BBL/4-1BB was blocked by anti-4-1BBL.The expressions of 4-1BB,CD40L,4-1BBL,CD40 and CD69 of T cell and B cell before blocked and after blocked by anti-4-1BBL were detected by flow cytometry.Results：The expression of CD69 on T cells of SLE patients showed significant difference（P0.01） with healthy controls.The expressions of 4-1BB,CD40L,4-1BBL and CD40 on the lymphocytes of SLE patients were significantly higher than that of normal control（P0.01）.There were obviously increased expression of 4-1BB and CD40L on the T lymphocytes stimulated by anti-CD3 antibody in SLE patients than healthy controls（P0.01）.With the anti-4-1BBL monoantibody blocked,there were decreased expression of 4-1BB CD40L（P0.01）.The expression of CD40 on B cell was significantly decreased,which is less than SLE unstimulted group and SLE stimulated group（P0.05）.Conclusions：T cell and B cell activation level of SLE patients were increased and 4-1BBL/4-1BB signals play a role in keeping the activation.

关 键 词：红斑狼疮 系统性 4-1BB CD40L CD69 

分 类 号：R593.24[医药卫生—内科学]