TK酶基因在乳酸乳球菌中的表达  

Expression of thymidine kinase (TK) gene in Lactococcus lactis

作　　者：阿荣[1,2] 孟祥晨[1,2] 王丽群[1,2] 

机构地区：[1]乳品科学教育部重点实验室,哈尔滨150030 [2]东北农业大学食品学院,哈尔滨150030

出　　处：《东北农业大学学报》2011年第11期14-18,共5页Journal of Northeast Agricultural University

基　　金：东北农业大学科技人才启动基金项目

摘　　要：利用乳酸乳球菌nisin诱导基因表达系统(the Nisin Controlled gene Expression system,NICE)表达TK酶基因。采用基因克隆和表达方法,表达产物通过SDS-PAGE进行鉴定。重组质粒测序结果表明,转化到乳酸乳球菌中的TK基因与pWSTK6基因(登录号为EU530625.1)相似度99%;同时,通过SDS-PAGE观察到乳酸乳球菌NZ9000表达的TK酶。成功构建的重组质粒pNZ8148-HSV1-TK能够在乳酸乳球菌NZ9000中稳定表达TK酶。Thymidine kinase gene was expressed in Lactococcus lactis by the nisin controlled gene expression system.Thymidine kinase was obtained by gene cloning and expressing,and the expressed products were identified by SDS-PAGE.The vector pNZ8148-HSV1-TK constructed in Lactococcus lactis NZ9000 was sequenced and blasted in NCBI,and the result showed that the sequencing of pNZ8148-HSV1TK had 99% similarity with that of Accession No.EU530625.1（Cloning vector pWSTK6）.In addition,a 41KD protein with the same size of TK protein secreted by Lactococcus lactis NZ9000 was gained through SDS-PAGE,which provided an evidence that the vector construction had been done.Thymidine kinase was able to be expressed steadily by the vector pNZ8148-HSV1-TK constructed in Lactococcus lactis NZ9000.

关 键 词：TK酶基因 载体构建 自杀基因治疗 乳酸乳球菌 

分 类 号：Q782[生物学—分子生物学]