微波消融治疗小鼠黑色素瘤及对肿瘤血管生成的影响  

Therapeutic effect of microwave ablation in mice bearing transplanted melanoma through inhibition of angiogenesis

作　　者：李纪强[1] 姜兆静[1] 张积仁[1] 

机构地区：[1]南方医科大学附属珠江医院肿瘤中心,广东广州510282

出　　处：《实用肿瘤杂志》2011年第6期569-572,共4页Journal of Practical Oncology

基　　金：卫生部科技专项基金(W2009BX015)

摘　　要：目的研究微波消融对小鼠黑色素瘤肿瘤负荷、生存期、肿瘤血管生成的影响。方法建立C57BL/6J小鼠B16-BL6黑色素瘤皮下移植模型,随机分成对照组、微波低剂量组、微波中剂量组、微波高剂量组,比较各组瘤重,病理学检测消融后肿瘤坏死、复发情况,免疫组织化学检测VEGF和CD34表达,计算微血管密度(microvesseldensity,MVD),记录其生存期。结果微波消融各组小鼠肿瘤重量较对照组显著减少(P<0.05);病理学观察发现消融病灶肿瘤细胞大量坏死,肿瘤血管破坏;消融后微血管密度MVD计数及血管内皮生长因子(vascularendothelial growth factor,VEGF)表达显著降低(P<0.05);但微波消融各组同对照组比较不具有生存期优势(P>0.05)。结论微波消融可抑制小鼠消融后黑色素瘤病灶血管新生,并减少肿瘤负荷,但并未延长小鼠生存期。Objective To investigate the therapeutic effect of microwave ablation（MA） in mice bearing transplanted melanoma.Methods C57BL/6J mice were subcutaneously inoculated with B16-BL6 cells.The transplanted colon tumor was treated with low,intermediate and high doses of MA.The tumor volume and recurrence were measured.The survival time of C57BL/6J mice was observed.Microvessel density（MVD） was analyzed by immunohistochemical staining of CD34 in transplanted tumors and the expression of vascular endothelial growth factor（VEGF） was also examined immunohistochemically.Results The tumor sizes in mice treated with MA were significantly smaller than those in model mice（P〈0.05） .Pathological examination revealed that there was tumor vascular destruction and no tumor recurrence in ablation lesions.VEGF expression and MVD were significantly reduced（P〈0.05） .There was no significant difference in survival time between the MA groups and the control group（P〉0.05） .Conclusion Microwave ablation can reduce tumor load by inhibiting angiogenesis of tumor,but may not prolong survival of tumor-bearing mice.

关 键 词：黑色素瘤/病理学 黑色素瘤/治疗 微波治疗应用 抗原 CD34/生物合成 内皮生长因子/生物合成 免疫组织化学 

分 类 号：R739.5[医药卫生—肿瘤]