乌司他丁对大鼠心肺复苏后早期血清S—100B蛋白和NSE的影响  

Influence of ulinastatin on serum S - IOOB protein and NSE in rats at the early stage after cardiopulmonary resuscitation

作　　者：杨晴[1] 李新宇[2] 王晓燕[2] 张莉[1] 秦怡[1] 

机构地区：[1]石河子大学医学院,新疆832002 [2]兰州军区乌鲁木齐总医院ICU,新疆乌鲁木齐830000

出　　处：《中国急救医学》2011年第12期1092-1095,I0013,共5页Chinese Journal of Critical Care Medicine

基　　金：天普基金（N0.01201040）

摘　　要：目的探讨乌司他丁（UTI）对大鼠心肺复苏（CPR）后早期血清S-100B蛋白、神经元特异性烯醇化酶（NSE）及海马病理改变的影响及作用。方法成年雄性SD大鼠随机分为假手术对照组、复苏组和UTI组，每组10只。采用窒息法致复苏组和UTI组大鼠心脏骤停（CA），继而进行心肺复苏（CPR）。UTI组于自主循环恢复（ROSC）后2min内经颈动脉推注注射用UTI100000U／kg，与生理盐水配伍成2mL溶液。对照组仅行麻醉、气管切开和血管穿刺。复苏组和UTI组于ROSC后1h，对照组于气管切开后1h经颈动脉取血和快速断头取左侧脑海马组织。采用双抗体夹心酶联免疫吸附法（ELISA）分别检测血清S-100B蛋白与NSE水平；光镜下观察海马的病理改变。结果与假手术对照组比较，复苏组和uTI组大鼠血清S-100B蛋白与NSE浓度在ROSC后1h均明显升高（P〈0．01）；与复苏组比较，UTI组大鼠血清S-100B蛋白浓度显著降低（P〈0．01），而NSE的浓度降低不明显（P〉0．05）。uTI组病理损害轻于复苏组。结论UTI能降低大鼠CPR后早期（1h）血清S-100B蛋白和NSE水平，减轻病理损伤，对CPR过程中的脑组织具有保护作用。Objective To investigate the effects of ulinastatin on serum concentration of S - 100B protein, NSE and the histopathological changes of hippocampus in rats at the early stage after cardiopulmonary resuscitation （CPR）. Methods male SD rats were randomly divided into three groups： sham - operation control group （n = 10）, resuscitation group （n = 10 ） and ulinastatin group （n= 10）. Asphyxiation cardiac arrest（CA） and CPR model was used in resuscitation group and ulinastatin group, with ulinastatin for injection 100 000 U/kg via arteria carotis within 2 minutes after restoration of spontaneous circulation （ROSC）. Anaesthesia, tracheotomy and vascular centesis were performed in rats without asphyxiation and CPR in sham - operation control group. Samples was taken at 1 h after tracheotomy or ROSC. Serum levels of S - 100B protein and NSE were evaluated by enzyme - linked immunosorbent assay （ELISA）. The pathological changes of hippocampus were observed under light microscope. Results Both S - 100B protein and NSE were significantly increased in serum at 1 h post ROSC compared with sham - operation control group （ P 〈 0.01 ）. The level of serum S - 100B protein decreased at 1 h after ROSC in ulinastatin group compared with resuscitation group （P 〈 0.01 ）. There were milder pathological changes in ulinastatin group than those in resuscitation group. Conclusion Ulinastatin could decrease the level of serum S - 100B protein and NSE, relieve the pathological changes significantly, which alleviate the brain injury in the early stage in rats after CPR

关 键 词：心肺复苏 S—100B蛋白 神经元特异性烯醇化酶 乌司他丁 脑损伤 

分 类 号：R651.15[医药卫生—外科学]