17β-雌二醇对6-OH多巴胺损伤的多巴胺能神经细胞的影响  

Effect of 17β-estradiol on injured dopaminergic neurons induced by 6-hydroxydopamine

作　　者：王晓舟[1] 刘亚萍[2] 王萌[3] 史子啸[3] 徐夏红[3] 王婷[3] 高殿帅[3] 

机构地区：[1]徐州医学院机能学实验中心,徐州221002 [2]徐州医学院形态学实验教学中心,徐州221002 [3]徐州医学院神经生物学实验中心,徐州221002

出　　处：《中华神经医学杂志》2011年第12期1219-1222,共4页Chinese Journal of Neuromedicine

基　　金：国家自然科学基金

摘　　要：目的研究17β-雌二醇（17β-E2）对6-羟基多巴胺（6—0HDA）所致的黑质多巴胺（DA）能神经细胞损伤的影响，探讨17β-E2是否具有神经保护作用。方法新生2～3d的雄性SD大鼠行常规中脑脑片培养，6．0HDA（200μmol／L）诱导损伤后分为实验对照组和17β-E2组，实验对照组继续常规培养，17β-E2组又分为1．0×10^-8mol/L、1．0×10^-9mol／L和1．0×10^1-mol/L 3个亚组，分别按上述浓度添加17β-E2进行干预。Westem blomng方法检测每组细胞干预15min、30min、60min后酪氨酸羟化酶（TH）的表达。结果1．0×10^-9mol/L 17β-E2组TH的表达最高，与实验对照组比较差异有统计学意义（P〈0．05）；增加或减少17β-E2的浓度均不能上调TH的表达；3个不同时间点中，17β-E2作用15min时，TH的表达最高，延长作用时间并不能增加TH的表达量。结论适当浓度（1．0×10^-9mol/L）17β-E2作用一定时间（15min）可以对受损的DA能神经细胞起到保护作用。Objective To study the effect of 17β-estrogen （17β-E2） on injured dopaminergic （DA） neurons induced by 6-hydroxydopamine （6-OHDA）, and explore whether 17β-E2 has neuroprotective effect. Methods The male SD rats aged 2-3 d were decapitated under sterile conditions; the brain slices were induced injury by 6-OHDA （200 μmol/L）; cells in the slices were divided into control group and 17β-E2 treatment groups （intervention with 1.0×10^-8 mol/L, 1.0×10^-9 mol/L and 1.0×10^10 mol/L of 17β-E2, respectively）. The tyrosine hydroxylase （TH） expression in DA neurons was observed by Western blotting at 15, 30 and 60 min after the intervention, Results The expression of TH in the 1.0×10^-9 mol/L 17β-E2 treatment group was the highest, which was significantly different as compared with that in the control group （P〈0.05）; increasing or decreasing the concentration of 17β-E2 could not regulate the expression of TH; in the 1.0×10^-9 mol/L 17β-E2 treatment group, TH expression at 15 rain after intervention enjoyed the highest level; the TH expression could not increase by extending the time. Conclusion The 17β-E2 treatment at certain concentration （1.0×10^-9 mol/L） for certain time （15 min） has a protective effect on injuried DA neurons

关 键 词：雌激素 多巴胺 酪氨酸羟化酶 

分 类 号：R742.5[医药卫生—神经病学与精神病学]