Increased leucine-rich repeats and immunoglobulin- like domains 1 expression enhances chemosensitivity in glioma  

作　　者：Baohui Liu Qianxue Chen Daofeng Tian Licluan Wu Junmin Wang Qiang Cai Heng Shen Baowei Ji Long Wang Shenqi Zhang Dong Ruan Xiaonan Zhu Zhentao Guo Huimin Dong Mingmin Yan 

机构地区：[1]Department of Neurosurgery, Renmin Hospital, Wuhan University, Wuhan 430060, Hubei Province, China [2]Department of Neurology, Renmin Hospital, Wuhan University, Wuhan 430060, Hubei Province, China

出　　处：《Neural Regeneration Research》2011年第32期2516-2520,共5页中国神经再生研究（英文版）

基　　金：the National Natural Science Foundation of China, No. 30973072;Independent Research Project of Wuhan University for Graduate Students, No. 201130202020001;Fundamental Research Funds for the Central Universities

摘　　要：Leucine-rich repeats and immunoglobulin-like domains 1 （LRIG1） is an anti-oncogene. LRIG1 is correlated with Bcl-2 in ependymomas. Decreased Bcl-2 and manganese superoxide dismutase expression can improve the chemosensitivity of glioma. In the present study, a tissue microarray of human brain astrocytomas was constructed. To investigate the relationship of LRIG1 with Bcl-2 and manganese superoxide dismutase, LRIG1, Bcl-2 and manganese superoxide dismutase expression in our tissue microarray was determined using immunohistochemistry. In addition, we constructed the LRIG1-U251 cell line, and its responses to doxorubicin and temozolomide were detected using the MTT assay. Results showed that LRIG1 expression was significantly negatively correlated with Bcl-2 and manganese superoxide dismutase expression in glioma. Also, proliferation of LRIG1-U251 cells exposed to doxorubicin or temozolomide was significantly inhibited, i.e. in the LRIG1-U251 cell line, the chemosensitivity to doxorubicin and temozolomide was increased. This indicates that increased LRIG1 expression produces a chemosensitivity in glioma.Leucine-rich repeats and immunoglobulin-like domains 1 （LRIG1） is an anti-oncogene. LRIG1 is correlated with Bcl-2 in ependymomas. Decreased Bcl-2 and manganese superoxide dismutase expression can improve the chemosensitivity of glioma. In the present study, a tissue microarray of human brain astrocytomas was constructed. To investigate the relationship of LRIG1 with Bcl-2 and manganese superoxide dismutase, LRIG1, Bcl-2 and manganese superoxide dismutase expression in our tissue microarray was determined using immunohistochemistry. In addition, we constructed the LRIG1-U251 cell line, and its responses to doxorubicin and temozolomide were detected using the MTT assay. Results showed that LRIG1 expression was significantly negatively correlated with Bcl-2 and manganese superoxide dismutase expression in glioma. Also, proliferation of LRIG1-U251 cells exposed to doxorubicin or temozolomide was significantly inhibited, i.e. in the LRIG1-U251 cell line, the chemosensitivity to doxorubicin and temozolomide was increased. This indicates that increased LRIG1 expression produces a chemosensitivity in glioma.

关 键 词：Leucine-rich repeats and immunoglobulin-like domains 1 astrocytoma CHEMOSENSITIVITY 

分 类 号：Q51[生物学—生物化学] S436.35[农业科学—农业昆虫与害虫防治]