高效液相色谱法测定化妆品中的保泰松  

Determination of Phenylbutazone in cosmetics by high performance liquid chromatography

作　　者：穆同娜[1] 王浩[1] 刘艳琴[1] 杨红梅[1] 史海良[1] 郭启雷[1] 

机构地区：[1]北京市海淀区产品质量监督检验所,北京100094

出　　处：《日用化学工业》2011年第6期459-461,共3页China Surfactant Detergent & Cosmetics

基　　金：国家质量监督检验检疫总局质检公益性行业科研专项资助项目(201010048)

摘　　要：建立了化妆品中保泰松的高效液相色谱分析法。采用甲醇超声提取样品,氨基固相小柱净化富集,以C18色谱柱(4.6 mm×250 mm,5μm)分离,流动相为20 mmol/L乙酸铵水溶液、甲醇和乙腈,流速1.0 mL/min,检测波长270 nm,柱温25℃,进样量10μL。该方法的检出限为2.0 mg/kg,线性范围0.2～100.0μg/mL,加标回收率86.1%～90.5%,相对标准偏差为5.79%～9.21%。A high performance liquid chromatography （ cosmetics was established. The sample was extracted HPLC ） method for determination of Phenylbutazone in by methanol with help of ultrasonic wave, followed by sample cleaning up and enriching with anino - solid - phase extraction cartridge, the HPLC separation was carried out on a Cls column （4. 6 mm × 250 mm,5 um）, with 20 mmol/L ammonium acetate solution, methanol and acetonitrile as mobile phase at a flow rate of 1.0 mL/min. The detecting wavelength is 270 nm; the column temperature is 25 ℃, and the sample injection volume is 10 uL. The limit of detection of the method achieves 2.0 mg/kg. The linear plots are displayed between 0. 2 and 100 ug/mL. Overall recovery achieves between 86. 1% and 90. 5% with RSD values between 5.79% and 9.21%.

关 键 词：化妆品 高效液相色谱法 固相萃取 保泰松 

分 类 号：O657.72[理学—分析化学] TQ658[理学—化学]