粗毛栓菌漆酶的诱导及其对中性染料和有机磷农药的降解  

Induce of laccase from Trametes gallica and its degradation on neutral dyes and organophosphorus pesticides.

作　　者：靖德军[1] 黄剑波[2] 杨洲平[1] 胡容[1] 程子彰[1] 黄乾明[1] 

机构地区：[1]四川农业大学生命科学与理学院,四川雅安625014 [2]四川农业大学动物医学院,四川雅安625014

出　　处：《应用生态学报》2011年第12期3300-3306,共7页Chinese Journal of Applied Ecology

基　　金：四川省教育厅重点科研项目(08ZA053);四川农业大学科技创新基金项目(00731200)资助

摘　　要：研究了不同初始培养pH和培养时间下,不同诱导剂对粗毛栓菌产漆酶诱导特性的影响及漆酶对中性染料、有机磷农药的降解.结果表明:RB-亮蓝、ABTS和邻联甲苯胺对粗毛栓菌产漆酶均有不同程度的诱导作用,其中ABTS的诱导作用最好,其诱导的最适初始pH为4.0,最佳时间为13d;所产漆酶的适宜反应温度为38℃,酶在40℃保温20min仍可保留78.6%的酶活力,稳定性较好;在ABTS介导下,漆酶降解6种中性染料的最佳温度分别为30℃(中性黑、中性枣红、中性桃红、甲基橙)和60℃(中性深黄、甲酚红),最适pH分别为6.0(中性黑)、2.0(中性枣红、中性桃红)和4.0(甲基橙、中性深黄、甲酚红),最佳降解时间分别为6h(甲基橙、中性深黄和甲酚红)、12h(中性桃红)和24h(中性枣红).而漆酶降解乐果、毒死蜱、敌百虫、哒嗪硫磷4种有机磷农药的最佳温度均为25℃,最佳降解时间为9h,最佳pH分别为10.0(乐果、毒死蜱、哒嗪硫磷)和8.0(敌百虫).The characteristics of the induction of laccase in Trametes gallica under different initial cultural pH,incubation time by different inducers were discussed,as well as the effects of temperature,pH and time on laccase degradation of six dyes and four organophosphors.The results showed that RB-bright blue,ABTS and o-toluidine affected the production of laccase at different levels,and ABTS was the best inductive agent in our test conditions,whose optimal initial pH and incubation time were 4.0 and 13 days,respectively.The appropriate reaction temperature of the laccase produced was 38 ℃,and it got a good stability,for it could retain 78.6% of the enzyme activity after 20 min holding at 40 ℃.Mediated by ABTS,the optimal temperature for laccase to degrade the six types of neutral dyes could be divided into two cases,that was 30 ℃（neutral black,neutral bordeaux,neutral pink,methyl orange） and 60 ℃（neutral dark yellow,cresol red）,the optimal pH were 6.0（neutral black）,2.0（neutral bordeaux,neutral pink） and 4.0（methyl orange,neutral dark yellow,cresol red）,respectively,while the optimal times separately were 6 h（methyl orange,neutral dark yellow,cresol red）,12 h（neutral pink） and 24 h（neutral bordeaux）.And using the same inductive agent,the best temperature for laccase to degrade dimethoate,chlorpyrifos,trichlorfon and parathion-pyridazine was 25 ℃,the suitable time was 9 h,and the optimal pH was 10.0 for dimethoate,chlorpyrifos and parathion-pyridazine,and 8.0 for trichlorfon.

关 键 词：粗毛栓菌 漆酶 难降解物 降解作用 

分 类 号：X172[环境科学与工程—环境科学] X592