液体或固体培养对环链棒束孢(Isaria cateinannulata)菌丝体中挥发性成分组成的影响  

Influence of liquid or solid culture conditions on the volatile components of mycelia of Isariacateinannulata

作　　者：张德龙[1] 王小董[1] 陆瑞利[1] 李康乐[1] 胡丰林[1] 

机构地区：[1]安徽省微生物防治省重点实验室,安徽农业大学,合肥230036

出　　处：《微生物学报》2011年第12期1616-1624,共9页Acta Microbiologica Sinica

基　　金：国家自然科学基金(30871676);安徽自然科学基金(11040606M69)~~

摘　　要：【目的】测定环链棒束孢(Isaria cateinannulata)在不同培养方式下,其菌丝中挥发性代谢物组成,分析代谢产物差异与培养方式间的相互联系。【方法】分别用SDAY培养基固体平板与SDY培养基液体摇瓶的培养方式进行培养;培养温度为25℃,培养周期为8天;用同时蒸馏萃取装置提取菌丝的挥发性成分;用气质联用仪进行数据采集及分析。【结果】固体培养菌丝体含有较多种类的挥发性成分,共鉴定41种化合物,其中酯类、醌类和肟类化合物为其特有。液体培养菌丝体含有的挥发性成分相对较少,共鉴定32种化合物,其中羧酸类化合物为其特有,同时酚类含量远高于固体菌丝。固体和液体培养菌丝体的主要挥发性成分都是烃类物质,其中烯烃类物质分别占固体培养菌丝和液体培养菌丝总挥发物的57.6%和7.85%,烷烃类物质分别占固体培养菌丝和液体培养菌丝总挥发物的9.19%和22.4%。【结论】固体或液体培养条件对环链棒束孢菌丝体中挥发性成分的组成有影响。[Objective]To determine the volatile components of mycelia of Isaria cateinannulata cultured under different culture conditions,and to analyze the relationships between the culture conditions and volatile metabolites.[Methods] Mycelia were cultured in solid plates with SDAY medium and liquid shake flasks with SDY medium.The culture conditions were at 25℃ and 8 days.Volatile components in the mycelia of I.cateinannulata were extracted with simultaneous distillation extraction and analyzed by gas chromatography-mass spectrometry.[Result] Alkenes,alkanes,heterocyclic and polycyclic aromatic hydrocarbons（PAH） were existed abundantly both in the mycelia of liquid and solid cultures,but the kinds and relative concentrations of the volatile components in mycelia of liquid and solid cultures were very different.Forty-one compounds were identified from the mycelia of solid culture and 32 compounds were identified from the mycelia of liquid culture.Esters,quinones and oximes were only found in solid cultured mycelia whereas carboxylic acids were only discovered in the mycelia of liquid culture.At the same time,mycelia of liquid culture contained much more phenols.The most abundant compounds in mycelia of liquid and solid cultures were hydrocarbons.The volatile extracts of solid cultured mycelia contained 57.6% alkenes and 9.19% alkanes.The volatile extracts of liquid cultured mycelia contained 7.85% alkenes and 22.4% alkanes.[Conclusion] Liquid or solid culture conditions influenced the volatile components of mycelia of I.cateinannulata.

关 键 词：环链棒束孢 液体培养 固体培养 菌丝体 气质联用 

分 类 号：Q93[生物学—微生物学]