吴茱萸碱对肥胖并发血管肥厚的作用研究  

The preventive effect of evodiamine on vascular hypertrophy in obese rats

作　　者：石海莲[1,2,3] 郑沁乐[4] 吴大正[1,3] 

机构地区：[1]上海中医药大学中药研究所,上海201203 [2]上海中医药大学龙华医院消化内科脾胃病研究所,上海201203 [3]上海中医药大学上海市复方中药重点实验室,上海201203 [4]上海中医药大学中药学院,上海201203

出　　处：《中国药理学通报》2011年第12期1687-1692,共6页Chinese Pharmacological Bulletin

基　　金：上海高校选拔培养优秀青年教师科研专项基金资助项目(No szy08042);上海市自然科学基金资助项目(No09ZR1431800);上海市复方中药重点实验室资助项目(No10DZ2270900)

摘　　要：目的研究吴茱萸碱对大鼠肥胖症及并发血管肥厚的预防作用。方法高脂饮食诱导肥胖大鼠模型;形态测量法动态观察大鼠体重、体长和Lee’s指数;ELISA法测定大鼠血清和组织降钙基因相关肽(CGRP)含量;免疫组化测定血管组织和背根神经节(DRG)TRPV1表达;比色法测定大鼠血清总胆固醇、低密度脂蛋白胆固醇、高密度脂蛋白胆固醇和甘油三酯含量。结果吴茱萸碱和辣椒素干预大鼠13周后,降低肥胖大鼠体重和Lee’s指数,降低肥胖大鼠内脏脂肪重量和内脏脂肪重量指数,并降低肥胖大鼠血清总胆固醇和甘油三酯含量。吴茱萸碱能逆转肥胖并发的血管肥厚;与正常大鼠相比,肥胖大鼠胸主动脉组织和DRG中TRPV1表达降低,血清和腹主动脉CGRP增加。吴茱萸碱和辣椒素能增加胸主动脉组织和DRG中TRPV1的表达;吴茱萸碱能降低血清和腹主动脉中CGRP含量。结论吴茱萸碱对高脂饮食诱导的肥胖具有良好的预防作用,其作用机制可能与调节血清、血管组织和DRG中CGRP和TRPV1的表达有关,其具体机制值得进一步的深入研究。Aim To study the preventive effect of evodiamine on vascular remodeling in obese rats.Methods Obese rats were induced by high-fat diet;body weight,body length,abdominal circumference,and Lee＇s index were measured per week.Serum and tissue calcitonin gene-related peptide（CGRP） contents were measured by ELISA method.Vascular tissue and dorsal root ganglia（DRG） TRPV1 expression was determined by immunohistochemistry method.Serum total cholesterol,low density lipoprotein cholesterol,high density lipoprotein cholesterol and triglyceride levelswere detected.Results Evodiamine and capsaicin could significantly decrease body weight,abdominal circumference,Lee＇s index,fat mass,serum total cholesterol and triglyceride in obese rats.Evodiamine obviously reduced vascular hypertrophy in obese rats.Compared with normal rats,the expression of TRPV1 in DRG and vascular tissue was decreased significantly,and CGRP levels in serum and abdominal aorta were significantly increased in obese rats,but decreased in DRG and thoracic aorta.Evodiamine and capsaicin could increase the expression of TRPV1 in DRG and vascular tissue.Evodiamine significantly decreased CGRP levels both in serum and abdominal aorta,also,increased CGRP contents both in DRG and thoracic aorta.Conclusions Evodiamine has a good preventive effect on vascular remodeling in high-fat diet-induced obesity,and the mechanisms may be related to regulation of CGRP and TRPV1 expression.However,the detailed mechanisms of evodiamine on vascular remodeling in obese rats should be studied further.

关 键 词：吴茱萸碱 肥胖症 CGRP TRPV1 背根神经节 血管肥厚 

分 类 号：R-332[医药卫生] R284.1