葛根素对糖尿病大鼠心功能及血清TNF-α水平的影响  

Effects of puerarin on the heart function and the level of serum TNF-α in diabetic rats

作　　者：张一弛[1,2] 解砚英[1,2] 牟艳玲[1,2] 裴可灵[1,2] 

机构地区：[1]山东省医学科学院药物研究所 [2]山东省罕少见病重点实验室,山东济南250062

出　　处：《中国药理学通报》2011年第12期1700-1704,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 30701022);山东省自然科学基金资助项目(No Y2007C156)

摘　　要：目的探讨葛根素(Pue)对糖尿病心肌病(DCM)大鼠心功能改变及血清中肿瘤坏死因子(TNF-α)水平的影响。方法 SD大鼠一次性腹腔注射链脲佐菌素(STZ)60 mg.kg-1,8周后建立实验性糖尿病心肌损伤模型。随机分为DCM模型组、葛根素小剂量组(50 mg.kg-1.d-1)和葛根素大剂量组(200 mg.kg-1.d-1),每组10只。每天腹腔注射1次,连续用药8周后评价各组的体重(BW)、空腹血糖(BFS)、总胆固醇(TC)、甘油三酯(TG)、乳酸脱氢酶(LDH)、谷草转氨酶(AST)、肌酸激酶(CK)、心肌型肌酸激酶同功酶(CK-MB)含量。小动物超声成像系统观测左心室舒张末期内径(LVIDd)、左心室收缩末期内径(LVIDs)、短轴缩短率(FS)及左心室射血分数(LVEF)的改变。酶联免疫吸附实验(ELISA)法测定TNF-α水平。结果与DCM模型组相比,①葛根素大剂量组体重明显升高(P<0.01),BFS、TC、TG及AST、LDH、CK、CK-MB均明显下降(P<0.01);②葛根素大剂量组LVIDd值及LVIDs值明显下降(P<0.01),FS值及LVEF值明显升高(P<0.01,P<0.05);③葛根素用药组TNF-α水平明显降低,以高剂量组最明显(P<0.01)。结论葛根素能明显改善糖尿病大鼠的心功能,其心肌保护作用可能与降低血中TNF-α水平有关。Aim To investigate effects of puerarin on the heart function and serum level of TNF-α in diabetic cardiomyopathy（DCM） rats.Methods Male Sprague-Dawley（SD） rats were intraperitonealy injected with streptozotocin（STZ,60 mg·kg-1） to establish DCM model.Then the model rats were randomly divided into diabetic group,low dose puerarin（50 mg·kg-1） and high dose puerarin（200 mg·kg-1）,10 rats in each group.Corresponding drugs were intraperitoneally injected once a day.The animals in normal control group and diabetic group were given equal sodium chloride.After 8 weeks,the effects of puerarin on the DCM were observed by echocardiography,including left ventricular internal diameter at end-systole（LVIDs）,left ventricular internal diastolic diameter（LVIDd）,ejection fraction（EF）,shortening fraction（FS）,and also by the detection of biochemical indices and myocardial enzymes,including fasting blood glucose（BFS）,total cholesterol（TC）,triglyceride（TG）,glutamic oxalacetic transaminase（AST）,lactate dehydrogenase（LDH）,creatine kinase（CK） and isoenzyme of creatine kinase（CK-MB）.TNF-α levels were also determined by enzyme linked immunosorbent assay（ELISA）method.Results Compared with the DCM group ① the body weight（BW） in high dose puerarin group significantly increased（P0.01）,and the levels of BFS,TC,TG,AST,LDH,CK and CK-MB declined（P0.01）;②Compared with the DCM group,LVIDd and LVIDs were ameliorated in the puerarin group especially in high dose group（P0.01）,while FS and LVEF had significant increased（P0.01,P0.05）.Low dose group was not significant.③ The serum levels of TNF-α were remarkably declined in high dose puerarin group compared with DCM group（P0.01）.Conclusion Puerarin exerts preventive and remedial effects on the diabetic myocardium and significantly improves heart function in DCM rats which may be related to reducing the serum level of TNF-α.

关 键 词：葛根素 糖尿病大鼠 糖尿病心肌病 心功能 TNF-Α 血糖 

分 类 号：R-332[医药卫生] R284.1