BMP-7对大鼠脑缺血/再灌注损伤后nestin和GFAP表达的影响  

Effect of bone morphogenetic protein-7 on expressions of nestin and GFAP in rats with cerebral ischemia reperfusion injury

作　　者：汝丽娟[1] 裴海涛[1] 郭云良[1] 

机构地区：[1]青岛大学医学院附属医院急诊神经科,山东青岛266003

出　　处：《中国药理学通报》2011年第12期1718-1723,共6页Chinese Pharmacological Bulletin

基　　金：山东省自然科学基金资助项目(No ZR2009CM121)

摘　　要：目的观察骨形态发生蛋白-7(BMP-7)对大鼠局灶性脑缺血/再灌注损伤后巢蛋白(nestin)和胶质纤维酸性蛋白(GFAP)表达的影响。方法成年♂SD大鼠40只,随机分为假手术组、对照组、BMP-7治疗组,每组10只。应用线栓法制备大脑中动脉阻塞2 h模型,治疗组尾静脉注射BMP-7(0.1 mg.kg-1)250μl,对照组和假手术组尾静脉注射等量的无菌注射用水。再灌注24h后行神经功能评分,2,3,5-三苯四氮唑(TTC)染色观察脑梗死体积,TUNEL法检测细胞凋亡,免疫组织化学方法测定nestin和GFAP表达。结果与对照组相比,BMP-7组大鼠神经功能评分(t=4.66,P<0.01)和脑梗死体积百分比降低(t=6.98,P<0.01),TUNEL阳性细胞数降低(P<0.01)。缺血侧脑组织nestin和GFAP表达增加(P<0.01)。结论 BMP-7可上调大鼠脑缺血/再灌注损伤后nestin和GFAP表达,促进神经再生,起神经保护作用。Aim To explore the effect of bone morphogenetic protein-7（BMP-7）on expressions of nestin and glial fiberillary acidic protein（GFAP）in brain tissue of rat after cerebral ischemia reperfusion injury.Methods Ten rats from 40 healthy adult male Sprague-Dawley rats received sham-operation.The other rats were subjected to middle cerebral artery occlusion（MCAO） for 2 hours.The 20 successfully modeled rats were equally randomized into the treatment group and control group.Rats in the treatment group were intervened with BMP-7（0.1 mg·kg-1） 250 μl via caudal vein injection,while rats in control group and sham-operation group were injected with equal volume of sterile water for injection.Neurological deficits were evaluated by Bederson′s method at 24 h after ischemia-reperfusion.The infarction volume of brain was investigated by 2,3,5-triphenyl tetrazolium chloride coloring.The neuronal apoptosis was assessed by TUNEL staining and the expression of nestin and GFAP of thethree groups were analyzed by immunohistochemistry method.Results The Bederson′s score（t=4.66,P0.01） and focus infarction（t=6.98,P0.01）were lower in BMP-7 treatment group than those in control group.Besides,the number of TUNEL positive cells in treatment group was lower than those in control group（P0.01）.Compared with the control group,the expression of nestin and GFAP enhanced in BMP-7 treatment group（P0.01）.Conclusion BMP-7 can up-regulate the expression of nestin and GFAP,and promote neural regeneration to protect animals against ischemia injury.

关 键 词：脑缺血 骨形态发生蛋白-7 凋亡 神经再生 巢蛋白 胶质纤维酸性蛋白 

分 类 号：R-332[医药卫生] R322.81