大鼠角膜接触镜的仿真研制及初步应用  

Simulation design and primary usage of corneal contact lens in rats

作　　者：李俊岭[1] 周小煦[1] 宋艳萍[2] 赵育新[2] 李娜[1] 吴建国[1] 

机构地区：[1]天津医科大学眼科中心,天津市300384 [2]广州军区武汉总医院眼科,湖北省武汉市430070

出　　处：《眼科新进展》2011年第12期1115-1117,共3页Recent Advances in Ophthalmology

基　　金：中国博士后基金资助(编号:20090450774);天津医科大学眼科中心博士启动基金资助(编号:2008003)~~

摘　　要：目的通过对大鼠眼球的机械仿真和光学仿真设计,研制一种角膜接触镜,用于观察大鼠眼底并进行玻璃体内注射操作。方法实验分2部分,第1部分:利用UG7.5软件设计成年SD大鼠眼球模型,再利用Light Tools7.0软件设计一种角膜接触镜,使之在手术显微镜下能够简便清晰地看清大鼠眼底;第2部分:20只健康成年SD大鼠随机分成A、B2组,每组10只鼠10眼,由同一人行玻璃体内注射骨髓间充质干细胞的操作,A组使用研制的角膜接触镜完成操作,B组利用传统的盖玻片压平角膜方法完成操作,记录眼底图像清晰程度,并比较实验完成所需时间和并发症出现情况。结果获得的大鼠眼球模型数据与国内外的结果近似,纤维塑料材质的接触镜轻便,适用于本实验内的全部动物眼。A、B两组均顺利完成实验。A组大鼠角膜搁置接触镜后,视网膜图像稳定清晰,操作者可双手协作,完成玻璃体内注射操作的时间仅需(192.50±22.51)s;B组大鼠的眼底图像不稳定,单手完成眼内操作,时间长达(446.90±41.11)s,两组间差异有统计学意义(t=-17.17,P<0.01),A组平均节省时间254.40s。A组无一例出现并发症,B组出现2例晶状体误扎伤,两组间差异无统计学意义(P=0.273)。结论使用UG7.5和Light Tools7.0仿真软件研制的角膜接触镜,可简便快捷地看清大鼠眼底,更重要的是实现了双手眼部操作,明显提高了动物实验的效果。Objective To develop a rat corneal contact lens basing on simulation design and observe its primary effect for intravitreal injection.Methods The experiment was divided into 2 parts.Part 1：After the ocular physical parameters being measured,an adult SD rat eyeball model was designed by UG software.Then,the corresponding corneal contact lens model was designed by Light Tools software and manufactured.Part 2：Twenty healthy adult SD rats treating intravitreal injection of bone marrow mesenchymal stem cells（BMMSC） were randomly divided into 2 groups,the new corneal contact lens was used in group A（10 rats,10 eyes） and traditional methods of coverslip flattening the cornea in group B（10 rats,10 eyes）.The experimental effect,complication and cost time were recorded and compared.All the operations were performed by the same person.Designed corneal contact lens was used in group A,and traditional cover glass was used to press cornea for completing operation in group B.Clear level of fundus image was recorded,the complete time and complications were compared between the two groups.Results The physical parameter of simulation-designed rat eyeball was similar to the results from foreign research works and the plastic contact lens was suit for all animals in this experiment.With the help of the new designed corneal contact lens,the posterior capsule of lens,vitreous and retina in rats were distinct and intravitreal injection was quickly achieved through two-hands assistance,the whole operation time cost（192.50±22.51）s in group A,whereas,the fundus images were not stable and the intravitreous injection was performed by single hand in group B,the operation time consumed（446.90±41.11）s.There was a significant difference（t=-17.17,P0.01）,and the time saved 254.40 s in group A.In additional,no complication occurred in group A,but 2 cases of lens injury occurred in group B,although there was no statistical difference（P=0.273）.Conclusions The corneal contact lens designed by UG 7.5 and Light Tools 7

关 键 词：仿真设计 角膜接触镜 大鼠 玻璃体内注射 

分 类 号：R772[医药卫生—眼科]