黑线仓鼠AVP基因部分序列的系统进化及结构分析  

PHYLETIC EVOLUTION AND STRUCTURE ANALYSIS OF PARTIAL SEQUENCE OF AVP GENE IN CRICETULUS BARABENSIS

作　　者：王永安[1] 卜凡莉[1] 徐金会[1] 薛慧良[1] 徐来祥[1] 

机构地区：[1]曲阜师范大学生命科学学院,山东曲阜273165

出　　处：《井冈山大学学报（自然科学版）》2011年第5期132-136,共5页Journal of Jinggangshan University (Natural Science)

基　　金：国家"973计划"科技项目(No.2007CB109104);山东省高校科技计划项目(J09LC18);山东省中青年科学家科研奖励基金项目(BS2010NY012)

摘　　要：根据GenBank中黑线仓鼠(Cricetulus barabensis)同源物种的AVPcDNA序列设计引物,利用RT-PCR技术得到了黑线仓鼠精氨酸加压素(AVP)基因部分cDNA序列,包括外显子2的全部序列和外显子1、3的部分序列,共433bp,并注册到GenBank(登录号:JN227681)。该段序列共编码143个氨基酸,二级结构预测显示片段中含较多的α螺旋。该序列与其他物种相应区域的比较结果表明其同源性为84%～92%,氨基酸序列同源性为82%～93%。系统进化分析结果与物种亲缘关系的远近一致,该序列的克隆为研究AVP的表达调控机制奠定了基础,将有助于AVP的作用机制的研究及功能分析,同时该cDNA序列可作为物种亲缘关系或遗传距离研究的理想标记。Primers were designed according to the A VP cDNA sequence of the homologous species of Cricetulus barabensis from the GenBank. Partial AFP cDNA sequence including complete sequence of exon 2 and partial sequence of exon 1 and 3, with total of 433 bp sequence, was obtained by RT-PCR technology, and was registered to GenBank （accession number： JN227681）. This sequence encodes 143 amino acids, the result of secondary structure prediction shows that the fragment contains more α-helix. Comparison with the other species with the corresponding region of sequence and amino acid homology showed that the homologous of this sequence arc 84% - 92% and 82% - 93% respectively. The result of the phylogenetic evolution analysis of AVP sequonce is consistent with the relative species relationship. Clarifying this; sequence laid a foundatior, for studying the expression and regulation of AVP. This sequence will contribute to the mechanisms and functional analysis of AVP, and the cDNA sequence of AVP could be used as an ideal marker to study the species relationships or hereditary distance.

关 键 词：黑线仓鼠 AVP 克隆 序列分析 系统进化 

分 类 号：Q74[生物学—分子生物学]