高寒香菊花黄酮固体脂质体制备工艺优化  

Optimization of preparation conditions of solid liposome of coreopsis flower flavonoids

作　　者：敬思群[1] 迪里努尔.阿布都热合曼 米热班古.木太力甫 

机构地区：[1]新疆大学生命科学与技术学院,新疆乌鲁木齐830046

出　　处：《中国酿造》2011年第11期50-53,共4页China Brewing

基　　金：新疆维吾尔自治区科技攻关(重大专项)(200731136-3)

摘　　要：采用乙醇注入法制备高寒香菊黄酮固体脂质体。以包封率为考察指标,通过单因素试验和正交试验,对壁材的配比,乳化剂的用量,冷冻保护剂甘露醇等工艺条件进行了优化。结果表明,最优制备工艺条件为壁材配比2∶1,乳化剂6%,冷冻保护剂3%。在此工艺条件下制得的高寒香菊花黄酮固体脂质体的包封率为39.69%。高寒香菊花黄酮固体脂质体外观为椭圆形,粒度多分布在450nm^500nm之间,高寒香菊花黄酮固体脂质体是大单室脂质体。The coreopsis flower flavonoids liposomes were prepared by ethanol injection method. The effects of wall material ratio, amount of manni- tol as a cryoprotectant on entrapment efficiency were investigated through single factor and orthogonal experiment. The results showed that the opti- mal preparation conditions of coreopsis flower flavonoids liposomes were obtained as follows： ratio of lecithin to cholesterol 2：1, amount of tween-20 6%, amount ofmannitol 3%. Under these optimum conditions, the entrapment efficiency was up to 39.69%. Coreopsis flower flavonoids solid lipo- somes was elliptical shaped, and its particle size was distributed between 450nm and 500nm. Coreopsis flower flavonoids solid lipid was a kind of large unilarnellarvesicles.

关 键 词：高寒香菊花黄酮 固体脂质体 乙醇注入法 制备条件 优化 

分 类 号：R282.71[医药卫生—中药学]