苦参素联合放射治疗对人卵巢癌 HO8910细胞的作用机制探讨  

The mechanism study of oxymatrine combined with radiotherapy on HO8910 cells of human ovarian cancer

作　　者：刘欣阳[1] 高洪泉[1] 祁艳[1] 曹丽梅[1] 郑宇[1] 张晓梅[1] 

机构地区：[1]牡丹江医学院附属红旗医院肿瘤科,157011

出　　处：《国际中医中药杂志》2011年第12期1080-1082,共3页International Journal of Traditional Chinese Medicine

基　　金：黑龙江省卫生厅科研课题（项目编号：2007-035）

摘　　要：目的 探讨苦参素联合放射治疗对人卵巢癌HO8910细胞体外生长的作用机制。方法 将标本分为4组，对照组加培养液；苦参素组加苦参素至终浓度为4 mg/ml；放疗组单一放疗，剂量为4 Gy；联合组先放疗4 Gy后，更换含苦参素4 mg/ml的培养液继续培养。各组孵育10、24、48、72 h。倒置显微镜下观察细胞形态学的变化；运用流式细胞术检测细胞凋亡率。结果 ①倒置显微镜下观察，与对照组比较，苦参素组、放疗组、联合组作用卵巢癌HO8910细胞24 h、48 h均可见细胞体积缩小、胞质浓缩，胞核浓聚的凋亡细胞。②流式细胞术结果表明，对照组、放疗组、苦参素组及联合组的人卵巢癌HO8910细胞于24 h时的细胞凋亡率分别为0.87%、3.43%、1.38%、27.38%，在细胞周期中均出现亚二倍体凋亡峰，联合组诱导卵巢癌HO8910细胞凋亡的峰值最高。结论 浓度为4 mg/ml的苦参素、剂量为4 Gy的放射治疗及两者联合均可诱导HO8910细胞凋亡，二者联合作用效果更明显。Objective To study the effect of oxymatrine（OMT）combined with radiotherapy on the growth of HO8910 cells of human ovarian cancer in vitro. Methods All human ovarian cancer samples were divided into four groups： control group, treated with culture solution; OMT group, treated with 4 mg/ml OMT; radiotherapy group, treated by 4 Gy radiotherapy; and combination group, firstly treated by 4Gy radiotherapy, and then cultured with 4 mg/ml OMT. Observed the changes of cell morphology by invert microscope at 10, 24, 48, and 72 hours respectively. Flow cytometry was adopted to detect cell apoptosis. Results ① invert microscope observation showed that compared with the control group, HO8910 cells demonstrated apoptosis of diminution in volume, thickening in cytoplasm, and gathering in nucleus in all the other three groups at 24 and 48 hours. ② Flow cytometry showed that apoptosis rate of HO8910 cells and cell population in G1 phase increased in the combination group, which were significantly higher than the other three groups （P〈0.05）. Conclusion Both OMT（4 mg/ml）and radiation（4Gy）can induce cell apoptosis, while the combination of them showed better therapeutic results.

关 键 词：卵巢癌 细胞凋亡 苦参素 放射治疗 

分 类 号：R737.31[医药卫生—肿瘤]