膜技术在酶法生产L-色氨酸中去除蛋白质和色素的应用研究  

Applied research of removing proteins and pigments in enzymatic production of L-tryptophan with membrane separation technology

作　　者：韦平和[1] 彭加平[1] 周锡樑[1] 

机构地区：[1]常州工程职业技术学院江苏省应用酶工程技术研究开发中心,江苏常州213164

出　　处：《中国生化药物杂志》2011年第6期421-425,共5页Chinese Journal of Biochemical Pharmaceutics

基　　金：常州市科技攻关项目(CE20100018);江苏省高校"青蓝工程"资助项目

摘　　要：目的用超滤-纳滤膜分离法去除L-色氨酸酶法转化液中的蛋白质和色素。方法 L-色氨酸转化液经适当处理后,输入超滤膜和纳滤膜设备,分别收集输出的清液和浓液,然后检测其透光率并用加入碱或乙醇方式,检查转化液中蛋白质、色素的去除效果。结果在转化液pH调节至5.5～6.0、温度控制在20～25℃的膜分离工艺条件下,经超滤-纳滤输出的清液,加入碱或乙醇无蛋白质析出,透光率达85%以上,比使用活性炭脱色处理的滤液高2.7倍。结论膜分离法去除转化液中蛋白质和色素的效果明显优于传统的絮凝剂沉淀蛋白质和活性炭脱除色素,从而为酶法生产L-色氨酸提供了一条绿色、先进的纯化工艺。Purpose The ultrafiltration （UF） combined with nanofiltration （NF） membrane separation was used to remove proteins and pigments from enzymatic conversion solution of L-tryptophan. Methods The conversion solution of L-tryptophan with certain treatments was inputed into UF and NF membrane filtration equipments, and then clear filtrate and cloudy filtrate through these equipments were collected respectively. The efficiency of removing proteins and pigments from the conversion solution with membrane separation was examined by determining the transmittance of the filtrate and adding alkali or ethanol into the filtrate. Results The conversion solution was adjusted to pH 5.5-6.0, and feeding temper- ature was 20-25 ℃. Under these operating conditions,no proteins were precipitated from the clear filtrate by adding alkali or ethanol. The transmittance of the clear filtrate at 460 nm was above 85% which was of 2.7 times as high as that of activated carbon decolorization. Conclusion The efficiency of membrane sep- aration for removing proteins and pigments from the conversion solution was obviously better than tradi- tional flocculation precipitation and activated carbon decolorization used in the experiment. It developed a green and advanced purification process for enzymatic production of L-tryptophan.

关 键 词：膜分离 L-色氨酸 超滤 纳滤 蛋白质 色素 

分 类 号：TQ464.7[化学工程—制药化工]