嗜酸乳杆菌β-半乳糖苷酶基因表达载体的构建与蛋白特性分析  

Construction of β-galactosidase gene expression vector and analysis of protein characteristics

作　　者：邓红艳[1] 贺松[1] 赫兰辉[1] 

机构地区：[1]成都大学附属医院检验科,四川610081

出　　处：《国际检验医学杂志》2011年第19期2192-2193,共2页International Journal of Laboratory Medicine

摘　　要：目的构建嗜酸乳杆菌β-半乳糖苷酶基因表达载体并分析其表达蛋白的特性和结构。方法从嗜酸乳杆菌ATCC4356中克隆lacZ基因并构建表达载体pMG36e-lacZ,并对构建后的表达载体进行鉴定和表达蛋白进行二维与三维结构分析。结果成功构建了嗜酸乳杆菌ATCC4356-lacZ基因表达载体并分析出了表达的β-半乳糖苷酶二维和三维结构图。结论表达载体的构建和蛋白特性与结构分析结果为后续的蛋白表达和酶生物学特性研究奠定了基础。Objective To construct the β-galactosidase gene expression vector and study its protein characteristics and structure from I.actobacillus aeidophilus. Methods The lacZ gene was cloned from L. acidophilus ATCC4356 and was inserted into recombinant vector pMG36e lacZ,then the recombinant vector was identified and the expressed β-galactosidase was analyzed for 2D and 3D structure. Results Restriction enzyme digestion,PCR identification and DNA sequencing analysis confirmed that the construction of pMG36e-lacZ was successful,and the 2D and 3D structure of β-galactosidase from L. acidophilus ATCC4356 were got. Conclusion This research could provide a foundation for the subsequent research of protein expression and biological characteristics of β-galactosidase from L. acidophilus ATCC4356.

关 键 词：β半乳糖苷酶类 嗜酸乳杆菌 LACZ基因 蛋白特性 

分 类 号：R346[医药卫生—基础医学]