中药川续断促进再生牙周对正畸力反应的研究  

Research of the traditional Chinese drug Dipsacus Asper Wall in promoting regeneration of the periodontal reaction on orthodontic force

作　　者：王磊[1,2] 张梅[3] 王旭霞 王媛[4] 张君[1,2,4] 

机构地区：[1]山东大学齐鲁医院口腔科 [2]山东大学口腔医学研究所,济南250012 [3]山东医学高等专科学校口腔医学系,济南250002 [4]山东大学口腔医学院正畸科,济南250012

出　　处：《山东大学学报（医学版）》2011年第11期18-20,24,共4页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金资助项目(Q2008C11)

摘　　要：目的研究中药川续断在大鼠牙周病牙周重建后的新生牙周组织改建中的作用机制及其对破骨细胞的影响。方法选取48只SPF级SD雌性大鼠,随机分为川续断组和对照组,每组24只,制作大鼠重度牙周病模型并利用釉基质蛋白进行牙周重建之后,建立大鼠正畸牙移动实验模型。川续断组根据大鼠体质量按照6 g/(kg.d)-1灌服川续断水煎剂,对照组灌服生理盐水3 mL/d,两组动物于正畸加力28 d后处死。分离大鼠上颌骨,测量上颌第一磨牙移动的距离,制作牙周组织切片,染色后光镜下观察,并进行统计学分析。结果川续断组牙移动距离明显大于对照组(P<0.05)。光镜下可见川续断组牙周组织中破骨细胞数目明显增多,与对照组相比差异有统计学意义(P<0.05)。结论在正畸牙移动过程中川续断水煎液能促进再生牙周的破骨细胞增殖和分化,促进再生牙槽骨吸收及其修复重建,有利于正畸牙移动。Objective To observe the effects of the traditional Chinese drug Dipsacus Asper Wall on the construction of new periodontal tissue,as well as the mechanism of osteoclast during orthodontic tooth movement in rats.Methods Forty-eight female 8-week-old SPF SD rats were selected.They were randomly divided into 2 groups： the Dipsacus Asper Wall group and control group.Severe periodontal disease models were established and enamel matrix protein was used for periodontal reconstruction.Then,the model for orthodontic tooth movement was established.3 mL of NS was drenched to the control group and the Dipsacus Asper Wall group was given 6g/kg per day Dipsacus Asper Wall decoction.The rats were executed 28 days later.Rat upper jaws were separated.The distances of the tooth movement were measured and slices from the periodontium of the maxillary first molars were observed under an optical microscope.Results The movement distance of the first molar was significantly larger in the Dipsacus Asper Wall group than that in the control group.The amount of osteoclast in Dipsacus Asper Wall group was significantly larger than that in the control group（P0.05）.Conclusions Dipsacus Asper Wall is useful for the proliferation and differentiation of the osteoclast in regenerated periodontal,and can promote regenerated alveolar bone resorption and restoration in orthodontic tooth movement.

关 键 词：川续断 牙周病 釉基质蛋白 牙移动 破骨细胞 

分 类 号：R783.5[医药卫生—口腔医学]