热休克蛋白70对脑出血大鼠神经细胞Bcl-2、Bax蛋白表达的影响  

Effects of heat shock protein 70 on expressions of the Bcl-2 and Bax proteins in nerve cells of rats with intra-cerebral hemorrhage

作　　者：唐茜茜[1] 袁倩[2] 崔元孝[1] 张清华[1] 郭章玉[1] 

机构地区：[1]山东大学附属省立医院神经内科,济南250021 [2]山东大学附属省立医院医务处,济南250021

出　　处：《山东大学学报（医学版）》2011年第11期40-43,60,共5页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金资助项目(Y2008C27;2011GG21821)

摘　　要：目的探讨热休克蛋白70(HSP70)对大鼠脑出血神经细胞凋亡相关基因Bcl-2、Bax蛋白表达的影响。方法将60只SD大鼠分为假手术组,脑出血组各30只,每组又分为6 h、12 h、1 d、2 d、3 d、7 d 6个亚组。假手术组向尾状核注射生理盐水2μL,脑出血组向大鼠尾状核注射2μLⅣ胶原酶,分别于各时间点处死大鼠取脑组织,采用免疫组织化学染色,检测各组大鼠脑出血周围HSP70、Bax、Bcl-2蛋白的表达并进行统计学处理。结果 HSP70、Bcl-2、Bax含量脑出血组较假手术组均明显增高(P<0.01)。HSP70表达在6 h出现,3 d达高峰,7 d表达减少。Bc-2的表达在6 h出现,在1 d时达高峰,第2天维持在高表达状态,随后表达逐渐减少。Bax的表达分别在12 h和3 d达高峰,在1 d时表达呈下降趋势,第7天表达逐渐减少。结论 HSP70高表达对出血脑组织具有明显的保护效应,其促进脑组织抗凋亡蛋白Bcl-2的表达,减少促凋亡蛋白Bax表达,增加Bcl-2/Bax比率,以此抑制脑组织细胞凋亡,从而发挥其对神经细胞的保护作用。Objective To investigate the protection of heat shock protein 70（HSP70） on the rat brain after experimental intra-cerebral hemorrhage and their effects on expression of Bcl-2 and Bax proteins of nerve cells.Methods 60 Wistar rats were randomly divided into the sham operated group（n=30）,and the itra-cerebral hemorrhage group（n=30）.Each group was divided into 6h,12h,1d,2d,3d and 7d groups according to time.2μL of normal saline was injected into the caudate nucleus in the sham operated group,and 2μL of collagenase Ⅳ was injected into the caudate nucleus in the intra-cerebral hemorrhage group.Rats were killed at each time point and then their brains were isolated.Expressions of HSP70,Bax and Bcl-2 proteins in the cerebral tissue at different times were detected by using immunohistochemistry.Results Expressions of HSP70,Bcl-2 and Bax in the itra-cerebral hemorrhage group were higher than those in the sham operated group（P0.01）.HSP70 appeared at 6h,reached a maximum on 3d,and gradually decreased on 7d.Bcl-2 expression appeared at 6h,reached the maximum on 1d,maintained a high state of expression on 2d,and then gradually decreased.The Bax expression reached the peak at 12h and 3d,showed a downward trend on 1d,and gradually decreased on 7d.Conclusions This study demonstrates that high expression of HSP70 has an obvious protective effect on the intracerebral hemorrhage rat model,and it promotes expression of the anti-apoptotic protein Bcl-2,reduces expression of the proapoptotic protein Bax,and increases the Bcl-2/Bax ratio,thus inhibiting brain cell apoptosis and exerting a protective effect on nerve cells.

关 键 词：热休克蛋白70 BAX蛋白 BCL-2蛋白 脑出血 

分 类 号：R743.34[医药卫生—神经病学与精神病学]