Expression and localization of paxillin in rat pancreas during development  

作　　者：Jing Guo Li-Jie Liu Li Yuan, Ning Wang Wei De 

机构地区：[1]Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China [2]Department of Physiology, Southeast University, Nanjing 210009, Jiangsu Province, China

出　　处：《World Journal of Gastroenterology》2011年第40期4479-4487,共9页世界胃肠病学杂志（英文版）

基　　金：Supported by The National Natural Science Foundation of China,Grant No.81070620;International Cooperation Project of Jiangsu Province,Grant No.BK2007117 and BZ2008062

摘　　要：AIM:To investigate the expression and localization of paxillin in rat pancreas during development. METHODS:Pancreata from Sprague Dawley rat fetuses, embryos, young animals, and adult animals were used in this study. Expression levels of paxillin in pancreata of different development stages were detected by reverse transcription polymerase chain reaction and Western blotting. To identify the cell location of paxillin in the developing rat pancreas, immunohistochemistry and double-immunofluorescent staining were performed using antibodies for specific cell markers and paxillin, respectively. RESULTS:The highest paxillin mRNA level was detected at E15.5 (embryo day 15.5) following a decrease in the later developmental periods (P < 0.05 vs E18.5, P0 and adult, respectively), and a progressively increased paxillin protein expression through the transition from E15.5 to adult was detected. The paxillin positive staining was mainly localized in rat islets of Langerhans at each stage tested during pancreas development. CONCLUSION:The dynamic expression of paxillin in rat pancreas from different stages indicates that paxillin might be involved in some aspects of pancreatic development.AIM： To investigate the expression and localization of paxillin in rat pancreas during development.METHODS： Pancreata from Sprague Dawley rat fe- tuses, embryos, young animals, and adult animals were used in this study. Expression levels of paxillin in pan- creata of different development stages were detected by reverse transcription polymerase chain reaction and Western blotting. To identify the cell location of paxillin in the developing rat pancreas, immunohistochemistry and double-immunofluorescent staining were per- formed using antibodies for specific cell markers and paxillin, respectively.RESULTS： The highest paxillin mRNA level was detected at E15.5 （embryo day 15.5） following a decrease in the later developmental periods （P 〈 0.05 vs E18.5, P0and adult, respectively）, and a progressively increased paxillin protein expression through the transition from E15.5 to adult was detected. The paxillin positive stain- ing was mainly localized in rat islets of Langerhans at each stage tested during pancreas development.CONCLUSION： The dynamic expression of paxillin in rat pancreas from different stages indicates that paxillin might be involved in some aspects of pancreatic devel- opment,

关 键 词：Pancreas development Islet remodeling PAXILLIN Cell adhesion MIGRATION 

分 类 号：Q813[生物学—生物工程] Q959.837