超滤膜截留-二氧化钛选择性富集-纳升级液相色谱-串联质谱法检测血清中内源性磷酸化肽  

Selective Enrichment of Endogenous Serum Phosphopeptides with Ultrafiltration Membrane Intercept-Titanium Dioxide Chromatography Coupled with Nano Ultra High Pressure Liquid Chromatography-Quadrupole-Time of Flight Mass Spectrometry

作　　者：邹丽娟[2] 李娟[2] 万慧慧[1] 李秀玲[1] 梁鑫淼[1] 

机构地区：[1]中国科学院大连化学物理研究所,中国科学院分离分析化学重点实验室,大连116023 [2]大连医科大学附属第二医院,大连116023

出　　处：《分析化学》2011年第12期1781-1786,共6页Chinese Journal of Analytical Chemistry

摘　　要：采用超滤膜(Ultrafiltration membrane)去除血清中的大量高丰度蛋白、DNA和RNA等大分子量干扰化合物,然后利用TiO2富集超滤膜滤过液中的磷酸化肽,结合纳升级超高压液相色谱-四极杆-飞行时间质谱(nano UPLC-Q-TOF)分离和检测技术,提高了内源性磷酸化肽的检测灵敏度;将本方法应用于肺癌患者血清中的内源性磷酸化肽分析,成功检测到18种内源性磷酸化肽。本方法实现了高灵敏度、高选择性血清中内源性磷酸化肽的富集和检测,为肿瘤生物标志物的发现奠定了方法学基础。A novel method coupled ultrafiltration membrane with TiO2 was developed to remove large amount high-molecular weight compounds and enrich endogenous phosphopeptides in serum.The enriched endogenous serum phosphopeptides were characterized by nano ultra-high pressure liquid chromatography-quadrupole-time of flight MS（nano UPLC-Q-TOF MS）.Up to 18 endogenous phosphopeptides could be successfully identified from the serum of lung cancer.The sensitivity and efficiency of serum phosphopeptides detection was improved by this established method,which might be contributed to novel tumor biomarker discovery.

关 键 词：超滤膜 TiO2富集 内源性磷酸化肽 NANO UPLC-Q-TOF 

分 类 号：R730.4[医药卫生—肿瘤] O657.63[医药卫生—临床医学]