p73及p53基因多态性与甘肃武威人群胃癌风险相关性研究  

Association of Genetic Polymorphisms of p73 Gene and p53 Gene with the Risk of Gastric Cancer in Wuwei Population

作　　者：张伟华[1] 郭凤霞[2] 刘玉琴[1] 谢小冬[3] 

机构地区：[1]甘肃省肿瘤医院,甘肃兰州730050 [2]甘肃农业大学生命科学学院,甘肃兰州730070 [3]兰州大学医学遗传学研究所,甘肃兰州730000

出　　处：《中国肿瘤》2011年第11期810-815,共6页China Cancer

基　　金：甘肃省科技厅自然科学基金(3ZS041-A25-013);甘肃省医科院资助项目(2007-11)

摘　　要：[目的]评价p73基因G4C14-to-A4T14双核苷酸多态性(p73 G4A DNP)和p53基因第72密码子单核苷酸多态性(p53 Arg72Pro SNP)与甘肃武威市人群胃癌高发风险及胃癌不同病理亚型的相关性。[方法]p73G4ADNP的基因分型采用两双相对引物多聚酶链式反应法,p53 Arg72Pro SNP基因分型采用PCR-RFLP法。[结果]共检查胃癌病例385例以及健康对照412人。胃癌组中弥漫型胃癌305例(79.22%),肠型胃癌80例(20.78%)。对照组p73AT/AT、AT/GC及GC/GC基因型的频率分别为28.15%、47.09%和24.76%;胃癌组分别为21.98%、45.04%和32.98%;以AT/AT作为指示物,胃癌组和弥漫型胃癌的GC/GC纯合子基因型频率均高于对照组,优势比(OR)分别为1.71(95%CI,1.16～2.51)和1.87(1.24～2.81)。对照组p53基因Pro/Pro、Pro/Arg以及Arg/Arg基因型的频率分别为27.18%、50.49%及22.33%;胃癌组则分别为21.82%、45.45%和32.73%;以Pro/Pro为指示物,胃癌组和弥漫型胃癌Arg/Arg基因型频率显著高于对照组,OR分别为1.83(95%CI,1.24～2.70)和2.25(95%CI,1.47～3.43)。[结论]携带p73 G4A GC/GC基因型或p53 Arg/Arg基因型可能会增加胃癌,尤其是弥漫性胃癌的发病风险。[Purpose] To evaluate the associations between p73 gene G4C14-to-A4T14,p53 Arg72Pro SNP and gastric cancer risk in Wuwei City,Gansu Province.[Methods] The genotyping of p73 gene G4A DNP was conducted by the polymerase chain reaction with the confronting two-pair primers（PCR-CTPP）.The genotyping of p53 codon 72 SNP was performed using PCR-RFLP.[Results] A total of 385 cases with gastric cancer and 412 healthy controls were investigated.There were 305（79.22%） diffuse-type cases and 80（20.78%） intestinal-type cases in gastric cancer group.The frequencies of p73 AT/AT,AT/GC and GC/GC were 28.15%,47.09% and 24.76% in control group and 21.98%,45.04% and 32.98% in GC group,respectively.Using the AT/AT as the referent,the GC/GC homozygous genotype frequency was higher in gastric cancer group,mainly in diffuse-type gastric cancer cases than that in control group.The ORs were 1.71（95% CI,1.16～2.51） and 1.87（1.24～2.81） respectively.The frequencies of p53 codon 72 Pro/Pro,Pro/Arg and Arg/Arg were 27.18%,50.49% and 22.33% in control group and 21.82%,45.45% and 32.73% in gastric cancer group,respectively.Using the Pro/Pro as referent,the Arg/Arg genotype frequencies in GC and diffuse-type gastric cancer cases were significantly higher than that in control group.The ORs were1.83（95% CI,1.24～2.70）,and 2.25（95% CI,1.47～3.43）.[Conclusions] Individuals with p73 G4A GC/GC homozygote or p53 gene codon 72 Arg/Arg genotype might increase the risk of gastric cancer,especially the diffuse-type gastric cancer.

关 键 词：P73 P53 多态性 胃癌 甘肃 

分 类 号：R730.231[医药卫生—肿瘤] R735.2[医药卫生—临床医学]