Electrochemical Determination of Reduced Glutathione at Multiwalled Carbon Nanotubes/Poly(bromocresol green) Modified Glassy Carbon Electrode  

作　　者：HOU Ju-ying AI Shi-yun 

机构地区：[1]College of Chemistry and Material Science, Shandong Agricultural University, Tai ＇an 271018, P. R. China

出　　处：《Chemical Research in Chinese Universities》2011年第6期934-938,共5页高等学校化学研究（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.20775044);the Natural Science Foundation of Shandong Province of China(No.Y2006B20)

摘　　要：A multiwalled carbon nanotubes/poly(bromocresol green) modfied glassy carbon electrode (MWNTs-PBG/GCE) was used to investigate the electrochemical behavior of reduced glutathione(GSH). GSH showed an irreversible oxidation process on MWNTs-PBG/GCE with an oxidation peak at 0.77 V(vs. SCE) in a phosphate buffer solution(pH=4.0). The kinetic parameters of the electrochemical behavior of GSH on MWNTs-PBG/GCE were calculated. Under the optimal conditions and with the help of amperometric method, a linear relationship was obtained between the oxidation peak current and GSH concentration in the range from 2×10-7 mol/L to 5×10-6 mol/L with the detection limit as 1×10-8 mol/L(signal-to-noise ratio of 3). The current reached the steady-state current within about 5 s. The modified electrode surface had very good reproducibility and stability.A multiwalled carbon nanotubes/poly（bromocresol green） modfied glassy carbon electrode （MWNTs-PBG/GCE） was used to investigate the electrochemical behavior of reduced glutathione（GSH）. GSH showed an irreversible oxidation process on MWNTs-PBG/GCE with an oxidation peak at 0.77 V（vs. SCE） in a phosphate buffer solution（pH=4.0）. The kinetic parameters of the electrochemical behavior of GSH on MWNTs-PBG/GCE were calculated. Under the optimal conditions and with the help of amperometric method, a linear relationship was obtained between the oxidation peak current and GSH concentration in the range from 2×10–7 mol/L to 5×10–6 mol/L with the detection limit as 1×10–8 mol/L（signal-to-noise ratio of 3）. The current reached the steady-state current within about 5 s. The modified electrode surface had very good reproducibility and stability.

关 键 词：Poly（bromocresol green） GLUTATHIONE ELECTRODE Multiwalled carbon nanotube 

分 类 号：O646.5[理学—物理化学] X503.225[理学—化学]