抑制素α、βA、βB亚基在乳腺和生殖系统肿瘤组织中的表达  

Immunohistochemical expression of inhibin α,β~A,β~B subunits in breast and reproductive system cancers

作　　者：王宏卫[1] 王霞[1] 夏辉明[1] 张东升[1] 高晓东[1] 李红乐[1] 鲁桂琛 孙颖 

机构地区：[1]河南医科大学病理生理学教研室,郑州4500522 [2]中国医学科学院药物研究所合成室,北京100050

出　　处：《河南医科大学学报》1999年第4期13-15,共3页Journal of Henan Medical University

基　　金：卫生部科学研究基金;河南省自然科学基金

摘　　要：目的：抑制素（ＩＮＨ） 单克隆抗体α、βＡ、βＢ亚基在生殖系统肿瘤组织中的免疫组化定位研究，并探讨ＩＮＨ与乳腺癌的关系。方法：应用免疫组化ＳＰ法，检测４ 例卵巢颗粒细胞瘤、４ 例睾丸支持细胞瘤，５ 例睾丸精原细胞瘤及４０ 例乳腺浸润性导管癌ＩＮＨα、βＡ、βＢ各亚基的表达。结果：卵巢颗粒细胞瘤、睾丸支持细胞瘤，睾丸精原细胞瘤各亚基皆呈阳性反应。４０ 例乳腺癌浸润性导管癌中ＩＮＨα、βＡ、βＢ阳性率分别为７２．５％ ，７７．５％ 和７０．０ ％ ，各亚基阳性率无明显差异（ Ｐ＞ ０．０５） 。结论：ＩＮＨ除在生殖细胞中阳性表达外，在乳腺癌表达阳性率超过７０ ％ 。Aims:To study the immunohitochemistry in cancer tissues related with using the INH antibodies which were made by ourselves and the relationship between INH and breast cancer.Methods: Expression of the INH's α,βA and βB subunits were detected by immunohistochemical SP method in 4 case of human ovarian granulosa cell tumours, 4 cases of testical leydig cell tumours, 5 cases of spermatogonia tumours, and 40 cases of breast cancers. Results:Each subunit of the INH had positive reaction in the ovarian granulosa cell tumours,the testical spermatognia tumours and the testical leydig cell tumours.In 40 samples of breast cancers, subunit positive rates were 72.5%,77.5% and 70%, respectively, and the positive rate of each INH subunit had no significant difference between them ( P >0.05).Conclusion: The INH's McAb prepared by ourselves were usefullness. We first found that the positive rate of INH subunit in breast cancer exceeded to 70%.

关 键 词：抑制素 单克隆抗体 乳腺肿瘤 免疫组织化学 

分 类 号：R737.903[医药卫生—肿瘤]