软组织平滑肌肉瘤中p16、细胞周期蛋白D1和pRb蛋白的表达及其意义  

The significance of p16, cyclin D1 and pRb expression in soft tissue leiomyosarcoma

作　　者：胥健敏[1] 杨光华[1] 步宏[1] 郭立新[1] 张世羽[1] 杨秀英[1] 

机构地区：[1]华西医科大学附属第一医院病理科,成都610041

出　　处：《中华病理学杂志》1999年第6期414-417,共4页Chinese Journal of Pathology

基　　金：国家自然科学基金!( 编号39670293)

摘　　要：目的　探讨软组织平滑肌肉瘤（ＬＭＳ） 中ｐ１６、细胞周期蛋白Ｄ１（ｃｙｃｌｉｎ Ｄ１） 和ｐＲｂ 的蛋白表达、相互关系及其意义。方法　应用免疫组化方法检测４２ 例ＬＭＳ患者组织中ｐ１６ 、ｃｙｃｌｉｎ Ｄ１ 和ｐＲｂ的蛋白表达情况。结果　４２ 例中ｐ１６、ｃｙｃｌｉｎ Ｄ１、ｐＲｂ 的总异常率为９７ ．６％ ，其中ｐ１６ 和ｐＲｂ 蛋白表达缺失分别为３５．７ ％ 和４７．６％ ，ｃｙｃｌｉｎ Ｄ１ 过表达５７．１％ 。ｃｙｃｌｉｎ Ｄ１ 在平滑肌瘤中也有５５％ 呈过表达。２０ 例ｐＲｂ 阴性的ＬＭＳ中，有１９ 例ｐ１６ 呈中至强阳性，１ 例弱阳性；而１５ 例ｐ１６ 阴性的ＬＭＳ中，１２ 例ｐＲｂ 中至强阳性，３ 例弱阳性。低分化组的ｐＲｂ 缺失率明显高于高分化组。结论　（１）ｐ１６ｃｙｃｌｉｎｐＲｂ通路异常与ＬＭＳ的发病机制密切相关；（２）ｐ１６、ｐＲｂ 蛋白缺失和ｃｙｃｌｉｎ Ｄ１ 过表达三者可单独或共同参与ＬＭＳ的形成；（３）ｐ１６ 与ｐＲｂ 表达呈明显的负相关关系；（４）ｃｙｃｌｉｎ Ｄ１ 过表达是常见的分子水平改变，且可能出现较早；（５）ｐＲｂ 表达与ＬＭＳ的分化程度呈负相关；（６）ｐ１６、ｐＲｂ 和ｃｙｃｌｉｎ Ｄ１ 的表达与肿瘤发生部位?Objective To evaluate the significance of p16, cyclin D1 and pRb expression in human soft tissue leiomyosarcoma (LMS) and the relationship among these three proteins. Methods 42 cases of LMS were studied for p16, cyclin D1 and pRb expression with immunohistochemical method. Results The total abnormality percentage of p16, cyclin D1 and pRb expression in all 42 cases of LMS was 97.6%. The negative rate of p16 and pRb protein expression was 35.7% and 47.6% respectively, and the overexpression rate of cyclin D1 was 57.1%. Overexpression of cyclin D1 was also noticed in 55% leiomyomas. In the 20 cases of LMS which were pRb negative, 19 cases were moderately or strongly positive and one faintly positive for p16. Contrastly, in the 15 cases of LMS negative to p16, 12 cases were moderately or strongly positive and 3 cases faintly positive for pRb. Higher rates of loss of pRb protein were observed in the low differentiated group than in the high differentiated one. Conclusions (1) The abnormality of p16 cyclin pRb pathway is highly related to the pathogenesis of LMS. (2) Loss of p16 or pRb and/or overexpression of cyclin D1 may be involved in the pathogenesis of LMS. (3) There is a negative reciprocity between p16 and pRb protein expression in LMS. (4) Overexpression of cyclin D1 is a common molecular alteration that may occur in the early stage. (5) Expression of pRb is inversely related to the differentiation degree of LMS. (6) The expression of the 3 proteins studied has no relation to tumor location, recurrence, invasion or metastasis.

关 键 词：平滑肌肉瘤 软组织肿瘤 LMS P16蛋白 PRB蛋白 

分 类 号：R738.602[医药卫生—肿瘤]