空泡型质子泵阻断剂Baf.A1抑制破骨样细胞体外骨吸收研究  

V-ATPase inhibitor baflomycine A1 inhibits bone resorption by osteoclast like cells

作　　者：赵宁侠[1] 于世凤[1] 

机构地区：[1]北京医科大学口腔医学院病理室,100081

出　　处：《中华病理学杂志》1999年第6期436-439,共4页Chinese Journal of Pathology

基　　金：国家自然科学基金!( 编号39830430)

摘　　要：目的　从骨吸收机制比较骨巨细胞瘤（ｇｉａｎｔｃｅｌｌｔｕｍｏｕｒ ｏｆ ｂｏｎｅ， ＧＣＴ） 中的破骨样细胞（ｏｓｔｅｏｃｌａｓｔｌｉｋｅ ｃｅｌｌ）和破骨细胞（ｏｓｔｅｏｃｌａｓｔ） ，明确参与破骨样细胞骨吸收的质子泵类型及其在骨吸收中的作用，进一步探讨破骨样细胞的特征和来源。方法　采用骨片与破骨样细胞体外共同培养的方法，观察破骨样细胞形成的骨吸收陷窝数和陷窝面积，比较线粒体型质子泵ＦＡＴＰａｓｅ 阻断剂Ｏｌｉｇｍｙｃｉｎｅ（Ｏｌｉｇ）和空泡型质子泵（Ｖａｃｕｏｌａｒ Ｈ＋ｔｒａｎｓｌｏｃａｔｉｎｇ ＡＴＰａｓｅ，ＶＡＴＰａｓｅ）阻断剂Ｂａｆｌｏｍｙｃｉｎｅ Ａ１（Ｂａｆ．Ａ１） 对破骨样细胞骨吸收的影响。结果　只有Ｂａｆ．Ａ１ 明显阻断破骨样细胞的体外骨吸收作用，而Ｏｌｉｇ 对其无影响。结论　破骨样细胞具有与破骨细胞相同的骨吸收机制，提示破骨样细胞可能是破骨细胞。Objective In order to compare the role of osteoclast like cells (OLCs) originating from giant cell tumor of bone and the osteoclasts (OCs) on bone resorption, as well as to study which kind of proton pump is associated with bone resorption by the OLCs and its role related. Methods Culture of OLCs together with the bone slices, the number of pits and the surface areas formed by OLCs was counted. The effects of F0F1 ATPase inhibitor, oligmycine V ATPase inhibitor, baflomycine of OLCs on bone resorption were compared. Results Baflomycine are considered effective in inhibiting bone resorption but no definited effect obtained with oligmycine. Conclusions OLCs bear the similar bone resorption mechanism as that of OCs. It's considered that OLCs and OCs are derived from the same origin.

关 键 词：骨肿瘤 破骨细胞 骨吸收 GCT Baf.A1 

分 类 号：R738.102[医药卫生—肿瘤]