表达Angiostatin基因的B16黑色素瘤体内抑制作用  

Inhibitory Efficiency on Growth in vivo of B16 Melanoma Cell Expressing Angiostatin

作　　者：夏国宏[1] 卢卫新[1] 邢力[1] 费俭[1] 郭礼和[1] 

机构地区：[1]中国科学院上海细胞生物学研究所,上海200031

出　　处：《中国肿瘤生物治疗杂志》1999年第4期284-287,共4页Chinese Journal of Cancer Biotherapy

摘　　要：目的:研究表达Angiostatin基因的B16黑色素瘤的体内生长行为.方法:通过改造Plasminogen cDNA得到Angiostatin基因,构建成真核表达载体pAGAGS3后导入B16黑色素瘤细胞使其表达.结果:表达Angiostatin的B16黑色素瘤细胞体外生长速率和软琼脂中克隆形成能力均未改变.但接种小鼠26d后,体内生长抑制率达87%(P<0.01).结论:表达Angiostatin的B16黑色素瘤体内生长受到显著抑制.Objective: Study on the growth character of B16 melanoma cell which can express angiostatin. Methods: Angiostatin gene was constructed from human plasminogen cDNA by deletion mutation. A B16 melanoma cell clone named BAG28 which stably expresses angiostatin was established by introducing gene into it. Results: BAG28 in vitro had no changes in proliferation rate and the ability of clone formation in soft agar. Study in vitro showed that the tumor weight had reduced about 87% ( P < 0. 01 ) 26 days after inoculation in mouse. Conclusion: The growth of B16 melanoma cells expressing angiostain was significandy inhibited in vivo.

关 键 词：B16黑色素瘤 ANGIOSTATIN 基因表达 血管生成 

分 类 号：R739.5[医药卫生—肿瘤] R969.3[医药卫生—临床医学]