利用ISSR标记对恩施州茶树种质资源亲缘关系鉴定的研究  

Identification of Tea Germplasm Genetic Relationship in Enshi by ISSR Markers

作　　者：李卫东[1] 崔清梅[1] 张强[1] 侯伟华[1] 袁程晓[1] 梁金波[1] 戴居会[1] 

机构地区：[1]恩施州农业科学院,湖北恩施445000

出　　处：《黑龙江农业科学》2011年第12期7-9,共3页Heilongjiang Agricultural Sciences

基　　金：湖北省农业科技创新中心资助项目(2007-620-001-03)

摘　　要：为更好地收集、保存和研究茶树品种资源,从2008年开始应用ISSR分子标记技术,对恩施州茶树资源的亲缘关系和遗传多样性进行了研究。结果表明:采用CTAB法提取DNA,探索出茶树PCR扩增效果较好的反应体系及反应程序,筛选出28条左右多态性较高的ISSR引物,用类平均法进行聚类分析,得出供试品种遗传关系树状图。基于遗传相似系数的UPGMA聚类图中,在遗传相似性系数为0.64处,183份茶树种质资源被划分为两大类。For the purpose of collecting,conserving and researching tea germplasm,the relationship and genetic diversity of Enshi local tea germplasm have been studied since 2008 by ISSR markers.The results showed that reaction system and response procedures with good PCR amplification effect of the tea tree were explored using CTAB law to extract DNA,28 about higher ISSR polymorphism primer were selected,the genetic relationship phylogenetic tree of the tested varieties was obtained by an average of clustering analysis method.The results showed that based on the genetic similarity coefficient groups by UPGMA cluster figure,at the genetic similarity coefficient was 0.64 place,183 tea tree germplasm resources were divided into two categories.

关 键 词：恩施茶树 种质资源 ISSR 亲缘关系 

分 类 号：S571.1[农业科学—茶叶生产加工]