致痫剂马桑内酯引起培养的大鼠皮层神经细胞凋亡  

CORIARIA LACTONE, AN EPILEPTOGENIC AGENT INDUCES APOPTOSIS OF CULTURED CORTICAL NEURONAL CELLS OF FETAL RATS

作　　者：何旦莎[1] 郑德枢[1] 邓维意[1] 夏学峰[1] 

机构地区：[1]广州医学院神经科学研究所,广州510182

出　　处：《神经解剖学杂志》1999年第4期369-372,共4页Chinese Journal of Neuroanatomy

基　　金：卫生部立项广东省卫生厅1996 年度卫生部科研基金!资助项目(No. 11);广东省科委自然科学基金!资助项目(No. 960082

摘　　要：用有致痫作用的马桑内酯处理培养的大鼠皮层神经细胞。以原位ＤＮＡ 断端标记法检测培养神经细胞的凋亡，配以培养液中乳酸脱氢酶的检测，观察马桑内酯对培养皮层神经细胞的毒性作用，并与已知的神经毒剂海人酸作比较。结果显示，马桑内酯可诱导培养大鼠皮层神经细胞凋亡，而引起细胞坏死的作用不如海人酸明显，其对培养大鼠皮层神经细胞的神经毒作用以导致神经细胞凋亡为主。Apoptosis of cultured cortical neuronal cells of fetal rats was detected with in situ TUNEL assay, and colorimetric LDH assay was used to determine the neurotoxic activity of coriaria lactone(CL), a definitive epileptogenic agent, and compared with that of a known neurotoxicant kainic acid (KA). Results showed that CL can induce apoptosis on cultured cortical neuronal cells, but necrosis is not so obvious as compared to that of KA. This suggests that apoptosis is the major event involved in CL cytotoxicity in cultured cortical neuronal cell. (Figures 2～9 on plate 64)

关 键 词：马桑内酯 神经毒性 凋亡 神经细胞 癫痫 大鼠 

分 类 号：R742.102[医药卫生—神经病学与精神病学] R996.2[医药卫生—临床医学]