从EDN1和NOS2A基因筛选与有氧训练效果相关分子标记的研究  

Research on Genetic Markers for Aerobic Training Response in EDN1 and NOS2A Genes

作　　者：张漓[1] 李燕春[2] 聂晶[3] 胡扬[2] 衣龙彦[2] 

机构地区：[1]国家体育总局体育科学研究所,北京100061 [2]北京体育大学,北京100084 [3]江西师范大学,江西南昌330027

出　　处：《体育科学》2011年第12期62-72,共11页China Sport Science

基　　金：国家科技部资助项目(2003BA904B04)

摘　　要：目的:拟从EDN1及NOS2A基因的多态性位点中筛选出与有氧训练效果相关的分子标记,为帮助制定个性化的有氧耐力训练方案提供理论参考。方法:采用Association-Study研究方法,测定了102名中国北方汉族青年男性18周有氧训练前、后的有氧运动能力相关指标,包括通过递增负荷跑台运动和气体代谢仪测定最大摄氧量、无氧阈、跑节省化、肺功能,以及通过彩色多普勒测定的安静及三级固定负荷(50W→100W→150W)功率车运动3min后的心脏结构指标与功能指标等,分析上述指标的变化率与EDN1基因多态性(外显子SNP/rs5370、SNP/rs1800997和3’端SNP/rs4714383)、NOS2A基因多态性(启动子区STR(CCTTT)n和内含子SNP/rs2248814)的关联性。主要结果:1)经过长期有氧耐力训练后,EDN1基因SNP/rs5370的GT基因型人群在安静及运动状态下,左心室泵功能提高较GG基因型人群更为显著,SNP/rs1800997的3A/3A基因型人群左心室肥厚度增加率显著高于3A/4A基因型人群;2)NOS2A基因STR(CCTTT)n等位基因n值与耐力训练前的左心室射血分数及耐力训练后的心输出量、心搏指数提高程度显著正相关,与耐力训练前的最大肺通气量及耐力训练后的运动中肺通气量提高程度均显著正相关,且含有n>11的等位基因的基因型人群肺通气量改善显著优于两个等位基因n均≤11的基因型人群。结论:在我国北方汉族男性人群中,EDN1基因SNP/rs5370、rs1800997的GT、3A/3A基因型携带者进行长期有氧训练后,心功能提高效果优于GG、3A/4A基因型携带者。NOS2A基因STR(CCTTT)n的n>11等位基因携带者进行长期有氧训练后,肺通气功能与心功能提高效果显著优于两个等位基因n均≤11的基因型携带者,且有n值越高,训练效果越好的趋势。Objective： To study the association of 5 polymorphisms in EDN1 and NOS2A genes with aerobic training response in order to find genetic markers for predicting aerobic training effect. Methods：A cohort of 102 young men was submitted to an 18 weeks endurance training event. All of the subjects were North Han nationality of China and had no training back ground. The phenotype parameters of aerobic capacity and cardiac function were measured be fore and after endurance training. The aerobic capacity indexes including VO2 max, ventilation aerobic threshold, running economy and respiratory function. The cardiac function was detected under rest and during 3 fixed-loads exercise. Association was observed between the change of phenotype parameters and polymorphisms including SNP/rs5370, rs180097）7, rs4714383 in EDN1 gene and STR （CCTTT）n and SNP/rs2248814 in NOS2A gene. Main ResuLts： （1） After training, the increase of sedentary EDD and SV in EDN 1 SNP/rs5370 GT genolype was significantly higher than that in GG genotype. The increase of sedentary LVM in EDN1 SNP/ rs1800997 3A/3A genotype was significantly higher than that in 3A/4A genotype. （2） The n value of NOS2A STR （CCTTT）n showed significant positive linear correlation with VEmax before training and the increase of VEmax and VT VE after training. The n value of STR （CCTTT） n also exhibited significant positive linear correlation with EF during fixed load exer cise before training and the increase of SVI and CO during fixed load exercise after training. Furthermore, the increase of these indexes in n〉11 allele carriers was significantly higher than that in double n≤11 alleles carriers. Conclusion：Among men of Han Nationality in North Chi na, the C-T and 3A/3A genotypes carriers of SNP/rs5370 and rs1800997 in EDN1 gene can get better improvement of cardiac function from aerobic training than GG and ;3A/4A geno types carriers. The n〉11 allele carriers of STR（CCTTT）n in NOS2 A gene can get beter im provement of cardiopulmonary funct

关 键 词：内皮素-1基因 诱导型一氧化氮合酶基因 分子标记 基因多态性 有氧能力 心功能 

分 类 号：G804.7[文化科学—运动人体科学]