氧化修饰高密度脂蛋白对培养人动脉平滑肌细胞细胞周期蛋白D_1基因表达的影响  

Effect of Oxidatively Modified High Density Lipoprotein on Transcriptional Expression of Cyclin D_1 Gene in Cultured Human Arterial SMC

作　　者：江渝[1] 刘秉文[1] 覃扬[1] 孙芝琳[1] 

机构地区：[1]华西医科大学生物化学与分子生物学研究所,成都610041

出　　处：《中国生物化学与分子生物学报》1999年第6期978-982,共5页Chinese Journal of Biochemistry and Molecular Biology

基　　金：教育部博士点科学基金;美国纽约中华医学基金

摘　　要：动脉平滑肌细胞（ｓｍ ｏｏｔｈ ｍ ｕｓｃｌｅ ｃｅｌｌ，ＳＭＣ）是动脉粥样硬化（ａｔｈｅｒｏｓｃｌｅｒｏｓｉｓ，ＡＳ）斑块中的主要细胞，它的增殖在ＡＳ形成过程中极其重要．利用体外培养的人主动脉ＳＭＣ，观察了天然高密度脂蛋白（ｎａｔｉｖｅ ｈｉｇｈ ｄｅｎｓｉｔｙ ｌｉｐｏｐｒｏｔｅｉｎ，Ｎ－ＨＤＬ）及氧化修饰ＨＤＬ（ｏｘｉｄｉｚｅｄ ＨＤＬ，ＯＸ－ＨＤＬ）对培养人主动脉ＳＭＣ ｃｙｃｌｉｎ Ｄ１（细胞周期蛋白Ｄ１）基因转录表达的影响．结果表明：（１）Ｎ－ＨＤＬ对ＳＭＣｃｙｃｌｉｎ Ｄ１基因表达无影响（Ｐ＞ ０．０５）；（２）ＯＸ－ＨＤＬ使ＳＭＣｃｙｃｌｉｎ Ｄ１基因表达显著增强（Ｐ＜０．０１），其表达量随时间（２、１２、２４ ｈ）延长而增加．上述结果表明，ＯＸ－ＨＤＬ的致ＡＳ作用可能与其刺激ＳＭＣｃｙｃｌｉｎ Ｄ１基因表达增加有关．The smooth muscle cells (SMC) are the predominant type of cells within atherosclerotic lesions,and their proliferation plays an important role in the process of atherosclerosis (AS) genesis.Using the establishment of primary culture and subculture method for human arterial SMC,the effects of Native HDL and oxidized HDL on cyclin D 1 gene transcriptional expression were observed.Results are as follows:(1)N HDL had no effects on the expression of cyclin D 1 gene in the cultured human SMC( P >0.05);(2)OX HDL promoted the expression of cyclin D 1 gene significantly ( P <0.01),the expression increased with the SMC incubating time (2,12 and 24 hours)prolonged.These results suggest that the OX HDL's atherogenic role may be closely related to its stimulating effect on cyclin D 1 gene expression.

关 键 词：SMC HDL AS CYCLIN D1基因 氧化修饰 基因表达 

分 类 号：R543.502[医药卫生—心血管疾病]