阿魏菇多糖对荷瘤小鼠机体免疫功能的影响研究  

Effect of Polysaccharides from Pleurotus Ferulae on Immune System in Mutine Cervical Cancer U14

作　　者：巩平[1] 杨琳[2] 王冬慧[2] 黄伟[1] 王金辉[3] 

机构地区：[1]新疆石河子大学医学院第一附属医院肿瘤内科,新疆石河子832000 [2]新疆石河子大学医学院,新疆石河子832000 [3]石河子大学药学院省部共建教育部新疆特种植物药资源重点实验室,新疆石河子832000

出　　处：《辽宁中医杂志》2011年第12期2482-2484,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家"863"计划项目(20078AA10Z323)

摘　　要：目的:从免疫学角度探讨阿魏菇多糖抑制肿瘤的作用机理。方法:建立昆明小鼠宫颈癌U14移植瘤动物模型,随机分为模型对照组,顺铂作为阳性对照组,阿魏菇多糖组,阿魏菇多糖+顺铂组4组。分别给予不同的药物干预10天后,检测抑瘤率,同时观察阿魏菇多糖对小鼠免疫器官的影响;鸡红细胞半体内法观察阿魏菇多糖对小鼠腹腔巨噬细胞吞噬功能的影响;MTT法测定阿魏菇多糖对小鼠脾脏中NK细胞影响;淋巴细胞转化实验检测阿魏菇多糖对小鼠脾脏T、B淋巴细胞的影响。结果:阿魏菇多糖对小鼠宫颈癌U14移植瘤有抑制作用,抑瘤率达39%,可明显促进荷瘤小鼠腹腔巨噬细胞的吞噬功能,提高脾中NK细胞的免疫活性,增加脾指数,提高脾脏T、B淋巴细胞转化率,与顺铂联用时可提高顺铂对荷瘤鼠免疫功能的抑制。结论:阿魏菇多糖可通过提高小鼠机体免疫功能,抑制小鼠宫颈癌U14移植瘤的增殖。Objective：To investigate polysaccharides from Pleurotus ferulae（PFP） for their regulative effects on Immune System and murine uterocervical carcinoma number14（U14）.Methods：Animal tumor models have been established,then ramdomly divided them into 4 groups.Model group,DDP group,PFP group and PFP ＋ DDP group,given various treatment for 10 days;After treatment,the weight of the tumor was observed to evaluate the growth inhibitory effect of PFP.The effect of PFP on the proliferation and transformation of lymphocytes in spleen was determined by MTT assay,the effect of PFP on the killing activity of NK cell was determined by lactate dehydrogenase releasing assay.Mouse peritoneal macrophages phagocytosis was measured by red blood cell（semi-in vivo method） test.Results：The tumor inhibition rate was 39% after exposed.PFP promoted the proliferation of lymphocytes,the killing activity of NK cell and the function of macrophage.PFP could improve immunosuppression by DDP.Conclusion：PNMP isolated shows a marked inhibition effect on the U14 cells and improvement of immune function of the mice,which may be one of the antitumor mechanisms of PFP.

关 键 词：阿魏菇多糖 小鼠宫颈癌U14 免疫功能 

分 类 号：R-332[医药卫生]