在肾癌细胞系GRC-1细胞周期不同阶段Wnt-5A基因的表达情况  

Different Wnt-5A Gene Expression in Renal Cell Carcinoma GRC-1 Cell Line During Cell Cycles

作　　者：庄立岩[1] 张志文[2] 郭宏骞[1] 李宏军[1] 唐东起[1] 苑学礼[1] 刘漓波[1] 丁义[1] 郭应禄[1] 

机构地区：[1]北京医科大学泌尿外科研究所,北京100034 [2]北京医科大学生理学系,北京100083

出　　处：《生物化学与生物物理进展》1999年第6期603-605,共3页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金!(39870841)

摘　　要：Ｗｎｔ 基因所编码的蛋白质与许多生长因子一样具有分泌型生长因子的结构特点， 其家族成员Ｗｎｔ５Ａ 是许多恶性肿瘤的自分泌生长因子， 在肾细胞癌中表达显著升高． 为研究在细胞周期的不同阶段生长因子Ｗｎｔ５Ａ 在转录水平的表达情况， 我们采用胸腺嘧啶双阻断及高压笑气处理的方法， 使肾细胞癌细胞系ＧＲＣ１ 细胞同步化．用半定量反转录多聚酶链反应对处于细胞周期不同阶段的细胞ｃＤＮＡ 进行扩增， Ｓ期与Ｇ１ ， Ｍ 期Ｗｎｔ５Ａｍ ＲＮＡ表达存在差异显著（ Ｐ＜ ０－０５） ． 结果提示生长因子Ｗｎｔ５Ａ 在肾细胞癌的发生中具有潜在的作用， 在Ｓ 期作用可能尤为显著．To investigate the gene expression of growth factor Wnt 5A in different phases during cell cycle at transcriptional level.To synchronize the renal cell carcinoma GRC 1 cell line was by double thymidine blocks and high pressure N2O gas methods . Using Semi quantitative RT PCR (reverse transcriptase polymerase chain reaction) to amplify the cDNAs of Wnt 5A in different phases. Result showed that the mRNA expression of growth factor Wnt 5A was detected in RCC GRC 1cell line. In S phase, the highest level of Wnt 5A was observed, medial and lowest was in G1 and M phase, respectively. The differences between S and M stages were significant ( P <0 05).Therefore growth factor Wnt 5A has the potential effect on tumorigenesis, It contributes to all phases during cell cycle, but gives more influence to S phase during cell cycle.

关 键 词：WNT-5A 肾细胞癌 细胞周期 基因表达 RT-PCR 

分 类 号：R737.11[医药卫生—肿瘤] Q753[医药卫生—临床医学]