莲子叶淀粉质体和细胞质核糖体的蛋白质电泳分析  

Protein Electrophoretic Analysis of Amyloplast and Cytoplasm Ribosomes from Lotus Cotyledon

作　　者：孙德兰[1] 王红[1] 董云洲[2] 

机构地区：[1]中国科学院植物研究所,北京100093 [2]中国农业科学院生物技术研究中心,北京100081

出　　处：《Acta Botanica Sinica》1999年第12期1345-1347,共3页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家自然科学基金!(No .3 947730 3 74)资助项目&&

摘　　要：Amyloplastsandcytoplasmicribosomesincotyledoncellsoflotus (NelumbonucifevaGaertn .) havebeenobservedonthebasisofmorphology .Isolationoftheseribosomesbycentrifugationthrough 30 %to 55% (W /V)sucrosedensitygradientresultedinthreebandsofamyloplastsribosomesandfourbandsofcyto plasmicribosomes .TheauthorsusedtheseribosomesbandsforSDS_PAGEelectrophoresistoanalyseribosomes ofproteins .ThepatternsofSDS_PAGEbetweencytoplasmicribosomesofproteinsandamyloplastsribosomesof proteinsweredifferent.Theamyloplastsribosomesofproteinsshowed 2 6kDand 2 3kDbands ,andthecyto plasmicribosomesofproteinsshowed 6 5kDband .Theanalysisofelectrophoreticpatternsofthecytoplasmic ribosomesofproteinsshowedthattherewasanewlysynthesizedribosomesproteinwith 19kDmolecularweight in 18to 2 0daysafterfertilization .Amyloplastsandcytoplasmicribosomesincotyledoncellsoflotus (NelumbonucifevaGaertn .) havebeenobservedonthebasisofmorphology .Isolationoftheseribosomesbycentrifugationthrough 30 %to 55% (W /V)sucrosedensitygradientresultedinthreebandsofamyloplastsribosomesandfourbandsofcyto plasmicribosomes .TheauthorsusedtheseribosomesbandsforSDS_PAGEelectrophoresistoanalyseribosomes ofproteins .ThepatternsofSDS_PAGEbetweencytoplasmicribosomesofproteinsandamyloplastsribosomesof proteinsweredifferent.Theamyloplastsribosomesofproteinsshowed 2 6kDand 2 3kDbands ,andthecyto plasmicribosomesofproteinsshowed 6 5kDband .Theanalysisofelectrophoreticpatternsofthecytoplasmic ribosomesofproteinsshowedthattherewasanewlysynthesizedribosomesproteinwith 19kDmolecularweight in 18to 2 0daysafterfertilization .

关 键 词：淀粉质体 核糖体 蛋白质 SDS-PAGE 莲 细胞质 

分 类 号：Q244[生物学—细胞生物学] Q510.3