滋阴活血解毒超微中药方对脑缺血大鼠凝血酶原和蛋白酶激活受体-1 mRNA表达的影响  

Effect of nourishing yin improving blood circulation and detoxification ultrafine Chinese medicine on the expression of prothrombin mRNA and PAR-1 mRNA in cerebral infarction rats

作　　者：邓奕辉[1] 邓婧溪[2] 陈懿[3] 段春艳[2] 葛金文[4] 

机构地区：[1]湖南中医药大学中医内科学实验室,长沙410007 [2]湖南中医药大学,长沙410007 [3]湖南中医药大学中医学院生理教研室,长沙410208 [4]湖南中医药大学血管生物学实验室,长沙410208

出　　处：《中华中医药杂志》2011年第12期2816-2820,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：湖南省教育厅青年基金项目(No.08B005);湖南省自然科学基金资助项目(No.10JJ6047)~~

摘　　要：目的:观察滋阴活血解毒超微中药方对大鼠局灶性脑缺血后脑组织凝血酶原mRNA和蛋白酶激活受体-1(PAR-1)mRNA表达的影响。方法:24只大鼠随机分为假手术组、模型组、滋阴活血解毒超微中药方组和阿加曲班组,每组又分1、3d两个观察时相。用线栓法制备大脑中动脉闭塞(MCAO)模型。采用逆转录-聚合酶链反应(RT-PCR)法检测脑组织中凝血酶原mRNA及PAR-1 mRNA表达水平。结果:假手术组可见凝血酶原和PAR-1 mRNA微量表达;模型组、滋阴活血解毒超微中药方组和阿加曲班组脑组织凝血酶原和PAR-1 mRNA表达明显增强,与假手术组比较有显著性差异(P<0.01);但与模型组比较,滋阴活血解毒超微中药方组和阿加曲班组脑组织中凝血酶原和PAR-1 mRNA表达明显下降(P<0.01);治疗第1天后滋阴活血解毒超微中药方组凝血酶原和PAR-1 mRNA表达明显高于阿加曲班组(P<0.05),但第3d后PAR-1 mRNA表达两组无显著性差异。结论:滋阴活血解毒超微中药方和凝血酶抑制剂阿加曲班均能下调脑缺血模型大鼠脑内凝血酶原和PAR-1 mRNA的表达,减轻凝血酶的毒性损伤可能是滋阴活血解毒超微中药方发挥脑保护作用的机制之一。Objective： To observe the effect of nourishing yin improving blood circulation and detoxification ultrafine Chinese medicine prescription on the expression of prothrombim mRNA and protease-activated receptor-1（PAR-1） mRNA at brain tissue of focal cerebral infarction rats.Methods： Twenty-four rats were randomly divided into 4 groups： the sham-operated group,model group,Ziyin Huoxue Jiedu Ultrafine Chinese Medicine group（ZUG） and argatroban group（ABG）.Each group was divided into two time phase point of 1st day,3rd day.The model was reproduced by occluded middle cerebral artery（MCAO）.The expression of prothrombin mRNA and PAR-1 mRNA of brain tissue were detected by reverse transcription-polymerase chain reaction（RT-PCR） technique.Results： The low level of prothrombin mRNA and PAR-1 mRNA were detected in the sham-operated group.The expression of prothrombin mRNA and PAR-1 mRNA of brain tissue in model group,ZUG and ABG was significantly higher than that in the sham-operated group（P0.01）,and the expression of prothrombin mRNA and PAR-1 mRNA of brain tissue in ZUG and ATB was significantly lower than that in the model group（P0.01）;Compared with argatroban group,the expression of prothrombin mRNA and PAR-1 mRNA in ZUG increased obviously at first day（P0.05）,but no difference on PAR-1 mRNA at third day.Conclusion： nourishing yin improving blood circulation and detoxification ultrafine Chinese medicine and argatroban are effective to restrain the expression of prothrombin mRNA and PAR-1 mRNA of brain tissue.It may be one of cerebral protective effects of nourishing yin improving blood circulation and detoxification ultrafine Chinese medicine that reduce thrombin toxic injury.

关 键 词：滋阴活血解毒法 超微中药 局灶性脑缺血 凝血酶原 蛋白酶激活受体-1 

分 类 号：R285.5[医药卫生—中药学]