微波辅助提取孔鳐抗氧化和血管生成抑制活性软骨多糖的研究  

Microwave-assisted Extraction of the Polysaccharide with Antioxidant and Anti-angiogenesis Activity from the Cartilage of Raja Porosa

作　　者：罗红宇[1,2] 王斌[2] 冯刚[2] 曲有乐[2] 

机构地区：[1]华中科技大学生命科学与技术学院,武汉430074 [2]浙江海洋学院食品与药学学院,浙江舟山316004

出　　处：《中国食品学报》2011年第7期14-21,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(81001393);浙江省自然科学基金项目(Y2080579;Y2110636)

摘　　要：目的:对微波辅助碱液提取孔鳐软骨多糖的方法及其抗氧化和血管生成抑制活性进行研究。方法:采用微波辅助碱液提取与木瓜蛋白酶水解相结合的加工工艺,以正交试验法优化提取工艺参数。通过还原力、DPPH·和·OH清除实验对多糖抗氧化能力进行测定。采用鸡胚绒毛尿囊膜(CAM)模型对其血管生成抑制活性进行评价。结果:微波辅助碱液提取最佳工艺参数是:微波功率650W、提取温度60℃、碱质量分数15%、液料比35:1、提取时间7min。木瓜蛋白酶脱蛋白精制孔鳐软骨多糖的最佳条件是:酶解温度50℃,pH7.0,酶解时间2h,酶量1.0%。在此条件下精制的多糖为白色粉末,得率13.77%,纯度88.75%。在相同浓度下精制孔鳐软骨多糖抗氧化能力均大于粗提多糖。精制多糖的血管生成抑制活性与硫酸软骨素(CS)相当,并且呈剂量依赖关系。结论:微波辅助碱液提取和木瓜蛋白酶脱蛋白所得精制多糖具有很好的抗氧化和血管生成抑制活性。Objective：Processing technology and in vitro antioxidant and anti-angiogenesis properties of the polysaccharide from the cartilage of Raja porosa were investigated.Method：The optimal conditions of Microwave-assisted alkaline extraction（MAAE） and papain hydrolysis processes were explored using single-factor experiments and orthogonal array design with the extraction ratio of polysaccharide as index.The antioxidant activities of the polysaccharide were mea-sured through DPPH/hydroxyl radical-scavenging activity and reducing power test,and the anti-angiogenesis property was evaluated by inhibiting the formation of the blood vessels of the chick embryo chorioallantoic membrane（CAM）.Result：The optimal techniques obtained were 15% alkali extracting at 60 ℃ with microwave power 650 W,solvent to material ratio 35：1 and extraction time 7 min,the optimum conditions of enzymatic hydrolysis was temperature 50 ℃,pH 7.0,time 2 h and enzyme amount 1.0%.Under the optimal condition,the white powder of polysaccharide（RCP） was obtained at yield rate of 13.77% and the purity of 88.75%.Based on the experimental results,RCP showed strong in vitro antioxidant and anti-angiogenesis properties,and there was also a positive relation between the concentration and inhibitory effect.Conclusion：The MAAE method is a potential method for extraction polysaccharides from the cartilage of R.porosa.Thus,RCP has good potential as a natural antioxidant and angiogenesis inhibitor used in medicine industries.

关 键 词：孔鳐软骨 多糖 微波辅助提取 抗氧化 血管生成抑制活性 

分 类 号：TQ464.1[化学工程—制药化工]