固本抑癌方对Lewis肺癌kiss-1、nm23基因表达的影响  

Effects of Guben Yiai Recipe on Expression Levels of kiss-1 and nm23 in C57 Mice Bearing Lewis Lung Carcinoma Cell

作　　者：山广志 凌仕良 

机构地区：[1]浙江省宁波市中医院肿瘤科十五病区,浙江宁波315010

出　　处：《安徽中医学院学报》2011年第6期50-53,共4页Journal of Anhui Traditional Chinese Medical College

基　　金：浙江省中医药科技计划项目(2008CA095)

摘　　要：目的研究中药固本抑癌方对荷Lewis肺癌的C57小鼠瘤组织kiss-1、nm23基因表达的影响。方法以荷Lewis肺癌的近交系C57小鼠为模型,分为生理盐水组,固本抑癌方低、中、高剂量组,阿霉素组,阿霉素+固本抑癌方组,测定并比较各组肿瘤细胞kiss-1、nm23基因的表达水平。结果与生理盐水组比较,阿霉素+固本抑癌方组,阿霉素组,以及固本抑癌方中剂量组、高剂量组肿瘤细胞kiss-1、nm23基因的表达水平显著升高(P<0.05);且阿霉素+固本抑癌方组与阿霉素组,固本抑癌方中剂量组、高剂量组kiss-1、nm23基因的表达水平相比,差异具有统计学意义(P<0.05)。结论固本抑癌方能提高瘤组织kiss-1、nm23基因的表达水平,对肺癌转移具有一定的抑制作用。Objective To investigate the effects of GubenYiai Recipe,a traditional Chinese medicine prescription mainly composed of Taxus chinensis,Astragalus mongholicus,Chinese yam,semen coicis,Codonopsis pilosula,on the expression levels of suppressor genes kiss-1 and nm23 in the tumor tissues of C57 mice bearing Lewis lung carcinoma.Methods The C57 mice inbred strain bearing Lewis lung carcinoma was used as model.The mice were divided into normal saline treated group,high-,medium-,and low-dose of Guben Yiai Recipe treated group,and adriamycin plus Guben Yiai Recipe treated group.The expression levels of kiss-1 and nm23 in the tumor tissues were observed and compared among the six groups.Results Compared with those in normal saline treated group,the expression levels of kiss-1 and nm23 were significantly increased in adriamycin plus medium-dose Guben Yiai Recipe treated group,adriamycin treated group,and high-and medium-dose of Guben Yiai Recipe treated group.There was no significant difference in the expression levels of kiss-1 and nm23 between adriamycin plus medium-dose Guben Yiai Recipe treated group and any of the three treatment groups,i.e.,adriamycin treated group,high-dose Guben Yiai Recipe treated group,and medium-dose Guben Yiai Recipe treated group.Conclusion Guben Yiai Recipe possesses some inhibitive effects against the metastasis of lung cancer via increasing the expression levels of kiss-1 and nm23 in the tumor tissues.

关 键 词：肺癌转移 固本抑癌方 抑癌基因 KISS-1 NM23 

分 类 号：R734.2[医药卫生—肿瘤] R285.5[医药卫生—临床医学]