无精子因子微缺失及生殖激素水平在男性不育中的研究  

The analysis of AZF microdeletions and gonadal hormone level in men of infertility

作　　者：吴芳[1] 白晓红[1] 宋学茹[1] 杨琳[1] 

机构地区：[1]天津医科大学总医院生殖医学中心,天津300052

出　　处：《中国现代医学杂志》2011年第24期3024-3027,共4页China Journal of Modern Medicine

摘　　要：目的筛查不育男性(严重少精子症和无精子症患者)Y染色体无精子因子(AZF)微缺失的发生情况,同时探讨无精子因子微缺失患者的生殖激素水平。方法对212例严重少精子症和96例无精子症患者进行常规的Y染色体无精子因子微缺失的分析,并运用化学发光法测定生殖激素的水平。结果 308例患者中发现28例Y染色体微缺失患者,缺失率为9.09%,其中少精子症患者18例,无精子症患者10例。缺失类型包括AZFb微缺失1例(3.57%),AZFb+AZFc微缺失4例(14.29%),AZFc微缺失20例(71.43%),AZFc+AZFd微缺失2例(7.14%),AZFd微缺失1例(3.57%)。生殖激素的检测,Y染色体AZF微缺失患者的促卵泡生成素(FSH)值明显升高,差异有统计学意义(P<0.05)。结论 Y染色体AZF微缺失是男性不育的主要遗传因素之一,其中AZFc的缺失为常见的类型,对这些患者进行生殖激素水平的检测是必要的。【Objective】 Screening for AZF microdeletions in patients with infertility（severe oligospermia or azoospermia）,and exploring the levels of gonadal hormone about patients of Y chromosome microdeletion.【Methods】We regularly analyzed Y chromosome microdeletions among 212 patients with severe oligospermia and 96 patients with azoospermia,and detected the levels of gonadal hormone by chemiluminescent method.【Results】 Out of 308 patients,28 were found with Y chromosome microdeletion,with a rate of 9.09%,of which 18 were severe oligospermia,10 were azoospermia.All the deletion type were 1 case（3.57%） in AZFb,4 cases（14.29%） in AZFb＋AZFc,20 cases（71.43%） in AZFc,2 cases（7.14%） in AZFc＋AZFd,1 case（3.57%） in AZFd.In gonadal hormone testing,level of FSH in Y chromosome microdeletions groups was significantly increased（P 0.05）.【Conclusion】 Y chromosome AZF microdeletion is one of main genetic factors in infertile men,most of the deletions happen in AZFc.It is necessary to detect the level of gonadal hormone for these patients.

关 键 词：男性不育 Y染色体微缺失 无精子因子(AZF) 生殖激素 

分 类 号：R715.2[医药卫生—妇产科学]