连翘三萜类化合物对前列腺癌PC-3细胞增殖抑制及放疗敏感性的实验研究  

Effects of antiproliferation and radiosensitivity on PC-3 cell of prostate cancer induced by triterpenes component

作　　者：王彩莲[1] 殷海涛[1] 刘宝瑞[2] 

机构地区：[1]东南大学附属中大医院,南京210009 [2]南京大学医学院附属鼓楼医院

出　　处：《山东医药》2011年第45期25-27,共3页Shandong Medical Journal

基　　金：南京市医学科技发展项目(YKK09097)

摘　　要：目的探讨连翘三萜类化合物达玛-24-烯-3β-乙酰氧基-20s-醇(DM)对人前列腺癌PC-3细胞的增殖抑制及放疗增敏作用。方法采用流式细胞术检测不同浓度DM液对PC-3细胞周期、凋亡的影响;PC-3细胞接受6-mVX线照射,用克隆形成法检测细胞存活分数、计算放疗增敏比;端粒重复序列扩增法检测DM对PC-3细胞端粒酶活性的抑制作用;实时定量PCR测定DM对PC-3细胞周期相关基因表达的影响。结果 DM可诱导PC-3细胞凋亡,其作用6 h后肿瘤细胞端粒酶活性降低,48 h后渐恢复至正常。DM可使细胞周期相关基因p21、TGF-β、Smad3表达增加,Cyclin D1、CDC25A表达降低(P均<0.05)。DM有放疗增敏作用,其放疗增敏比为1.80。结论 DM可诱导PC-3细胞凋亡,抑制其细胞端粒酶活性,调节细胞周期相关基因表达,对前列腺癌PC-3细胞有放疗增敏作用。Objective To observe the effects of antiproliferation and radiosensitivity on PC-3 cell of prostate cancer induced by Dammar-24-ene-3β-acetate-20s-ol（DM）.Methods Cell cycles and apoptosis of PC-3 cell influenced by different concentrations of DM were detected with flow cytometry;the survival fractions of PC-3 cell were studied by colony-formation efficience after 6-mV X ray radiotherapy was administrated on PC-3 cell,and sensitization enhancement ratio was calculated;the inhibitive activities of telomerase of PC-3 cell influenced by DM were analyzed with combining telomeric repeat amplification protocol（TRAP）;the expression levels of cell cycle related genes of PC-3 interfered by DM were evaluated using real-time quantitative reverse-transcription PCR.Results DM can induce the apoptosis of PC-3 cell.The activities of telomerase of PC-3 cell may be reduced by DM after 6 hours and then returned to normal level after 48 hours.DM can increase the expression levels of cell cycle related genes p21,TGF-β and Smad3.However,the expression levels of Cyclin D1 and CDC25A were down-regulated by DM.DM had the effect of radiosensitivity.The sensitization enhancement ratio was 1.80.Conclusions DM may induce the apoptosis and inhibit the activities of telomerase of PC-3 cell and regulate the expression levels of cell cycle related genes.DM has the effect of radiosensitivity on PC-3 cell of prostate cancer.

关 键 词：前列腺肿瘤 PC-3细胞 达玛-24-烯-3β-乙酰氧基-20s-醇 放射治疗 

分 类 号：R737.25[医药卫生—肿瘤]