溶藻细菌的分离鉴定及对球形棕囊藻溶藻作用的环境因子研究  

Isolation and identification of algae-lysing bacteria and effect of environmental factors on Phaeocystis globosa

作　　者：赵玲[1] 李蔷[2] 尹平河[2] 杨晓新[2] 

机构地区：[1]暨南大学理工学院,广东广州510632 [2]暨南大学生命科学技术学院,广东广州510632

出　　处：《海洋环境科学》2011年第6期853-857,共5页Marine Environmental Science

基　　金：国家自然科学基金项目(41076068)

摘　　要：从珠海香洲码头赤潮海水中分离获得1株具有溶藻作用的细菌Y01,通过形态及16S rDNA序列分析鉴定为芽孢杆菌属。探讨了盐度、光照、藻细胞数等环境因子对球形棕囊藻溶藻效果的影响。结果表明:细菌Y01溶藻效果在盐度为30时优于其它盐度;光暗比(12 h∶12 h)时Y01的溶藻能力优于无光照条件,达到98%;利用HPLC进行藻细胞色素分析发现,随着棕囊藻细胞数的下降,藻细胞色素含量锐减,无光照条件下藻细胞色素的种类减少2种,分别是别藻黄素和叶黄素;Y01对处于生长初期的球形棕囊藻的抑制效果优于指数生长期的球形棕囊藻。The algicidal bacteria named Y01 was isolated from the red tide water in Xiangzhou shipside of Zhuhai.The strain Y01 was appraised as Bacillus sp through biophysics,biochemical and the analysis of its 16S rRNA sequence.To explore the suitable conditions of algae-lysing effect on Phaeocystis globosa by the isolated algae-lysis bacteria,the effects of salinity,illumination and algae density were studied.The results showed that it had the best inhibitory function at the salinity of 30.The algae-lysing ability under the condition of light cycles（12 h∶ 12 h） was better than total dark and the lytic rate was 98%,algal cytochrome reduced sharply followed the decline in algal density,alloxanthin and lutein were missed under the condition of total dark.The algae-lysing ability of Y01 bacteria to Phaeocystis in the early growth phase was better than that in the exponential growth phase.

关 键 词：球形棕囊藻 溶藻细菌 盐度 光照 藻细胞数 

分 类 号：X145[环境科学与工程—环境科学]