微阵列技术用于检测乳腺癌人表皮生长因子受体2基因状态  

Nuclei and tissue microarrays technique in detecting HER-2 gene expression in breast carcinoma

作　　者：蒋会勇[1] 马永权[1] 张雪峰[1] 

机构地区：[1]沈阳军区总医院普通外科,沈阳110840

出　　处：《中华乳腺病杂志（电子版）》2011年第5期9-12,共4页Chinese Journal of Breast Disease(Electronic Edition)

摘　　要：目的探讨细胞核微阵列技术及组织微阵列技术用于检测乳腺癌组织中HER-2基因扩增和蛋白表达状态。方法将248例乳腺癌普通组织蜡块制成组织微阵列组,应用免疫组织化学法(IHC)和荧光原位杂交法(FISH)分别检测HER-2基因和蛋白表达。两种检测方法的一致性采用检验,并以FISH检测结果为金标准,绘制IHC检测乳腺癌HER-2表达的ROC曲线图。结果 248例乳腺癌FISH与IHC检测结果一致性分析显示:Kappa=0.711,P=0.000,两种检测方法的一致性尚可。IHC检测乳腺癌HER-2ROC曲线下面积为0.888,P=0.000,约登指数为0.700,准确率为87.9%。IHC(++)中4例为17号染色体单体型,占FISH阳性病例总数的5.26%(4/76)。IHC(+++)中5例为17号染色体多倍体型,FISH检测均为阴性,占FISH阴性总例数的2.9%(5/172)。IHC的检测可以较好地反映出FISH的结果。结论细胞核微阵列及组织微阵列技术用于检测乳腺癌HER-2基因状态有着节约实验成本、同一性好、结果可靠的优点。Objective To investigate the usage of nuclei and tissue microarrays technique in detecting HER-2 gene amplification and protein expression in breast carcinoma. Methods Two hundred and forty-eight breast carcinoma paraffin embedded tissue blocks were used to construct tissue microarray groups. Fluorescence in situ hybridization （FISH） and immunohistochemistry （IHC） were used to detect the expressions of HER-2 gene and protein. The detection results of the two methods were analyzed using Kappa test. Taking the results obtained from FISH analysis as the gold standard, the ROC curve of IHC detecting HER-2 expression was drawn. Results The Kappa test of the two methods showed an acceptable consistency （ Kappa = 0. 711, P = 0. 000）. The area under the ROC curve of IHC detecting HER-2 expression was 0. 888 （P = 0. 000） , the Youden＇ s index was 0. 700 and an accuracy was 87.9%. Four of the IHC （＋ ＋） cases had haplotype of chromosome 17, with a positive rate of 5.26% （4/76） of the FISH positive cases. Five of the IHC（＋＋＋） cases had polyploid of chromosome 17, with a negative rate of 2.9% （5/172） of the FISH negative cases, indicating IHC results could better reflect FISH results. Conclusions Nuclei and tissue microarrays technique can be used in detecting HER-2 gene amplification and protein expression, and has the advantages of low cost and relatively reliable results.

关 键 词：组织微阵列 免疫组织化学法 荧光原位杂交 HER-2 乳腺肿瘤 

分 类 号：R737.9[医药卫生—肿瘤]