金属蛋白酶组织抑制因子1在乳腺癌组织中的表达及其与多种生物学指标的关系  

Expression of tissue inhibitor of metalloproteinase-1 in breast cancer tissue and its relationship with multiple biomarkers

作　　者：张绍坤[1] 郭冰[2] 王雷[1] 宁均[1] 周玉峰[1] 李春来[1] 

机构地区：[1]牡丹江市第一人民医院乳腺科,黑龙江牡丹江157011 [2]青岛市海慈医院,青岛266033

出　　处：《中华乳腺病杂志（电子版）》2011年第5期18-22,共5页Chinese Journal of Breast Disease(Electronic Edition)

基　　金：黑龙江省牡丹江市科技项目(G2010S0011)

摘　　要：目的研究乳腺癌组织中金属蛋白酶组织抑制因子1(TIMP-1)的表达及其与增殖细胞核抗原ki67、P53癌基因(P53gene)、人表皮生长因子受体-2(HER-2)表达的关系。方法应用免疫组织化学方法(SP法)检测104例乳腺癌和33例癌旁乳腺组织TIMP-1的表达情况以及乳腺癌组织Ki67、P53、HER-2的表达情况。非等级计数资料比较采用χ2检验,等级计数资料比较采用Mann-Whitney U或Kruskal-Wallis H非参数秩和检验。两指标间的相关性采用Spearman相关分析。结果 TIMP-1在乳腺癌组织中的阳性表达率为69.23%(72/104),在癌旁乳腺组织中的阳性表达率为15.15%(5/33),二者间差异有统计学意义(χ2=29.76,P=0.00)。TIMP-1表达与年龄、月经状态、手术方式、临床分期、肿瘤病理类型等因素无关(P>0.05),与肿瘤直径、组织学分级、淋巴结转移有关(P<0.01)。TIMP-1阳性表达与Ki67表达呈正相关(r=0.28,P<0.01),与P53表达呈负相关(r=-0.42,P<0.01),与HER-2表达不相关(r=-0.01,P>0.05)。结论 TIMP-1在乳腺癌组织中表达增高,且与乳腺癌侵袭过程关系密切;TIMP-1与多种生物学指标联合检测能更准确地预测预后、指导治疗。Objective To investigate the expression of tissue inhibitor of metalloproteinase-1 in breast cancer tissue and its relationship with Ki67, P53 and HER-2. Methods The immunohistochemistry method was used to detect the expressions of TIMP-1, Ki67, P53 and HER-2 in the tissues of 104 breast cancer specimens and 33 pericancer specimens. χ2 test was used to analyze the unranked qualitative data. Mann- Whitney U test or Kruskal-Wallis H test was used for comparison between ranked qualitative data. Spearman rank correlation test was conducted for analysis of correlation of TIMP-1 with Ki67, P53 and HER-2 in breast cancer. Results The positive expression rate of TIMP-1 was 69.23% （72/104） in cancer tissue, and 15.15% （5/33） in pericancer tissue, with significant difference between them （ χ2 = 29.76, P = 0.00）. The positive expression of TIMP-I was not related with age, menstrual status, operation modes, clinical stage, and pathological type of cancer （P〉0.05）, but correlated with tumor size, histological grades and lymph node metastasis （P〈0.01）. The positive expression of TIMP-1 was positively correlated with Ki67 （r = 0.28, P〈 0.01 ）, negatively correlated with p53 （ r = -0.42, P〈0.01 ）, and not correlated with HER-2 （ r = -0.01, P〉 0.05）. Conclusion The high expression of TIMP-1 in breast cancer tissue is highly correlated with invasion. Detection of TIMP-1 combined with other biomarkers can accurately indicate the outcome and instruct treatment for breast cancer.

关 键 词：乳腺肿瘤 金属蛋白酶类 金属蛋白酶1组织抑制剂 免疫组织化学 

分 类 号：R737.9[医药卫生—肿瘤]