Preliminary Biocompatibility Evaluation of Regenerated Antheraea yamamai Silk Fibroin in vitro  

作　　者：王建南 

机构地区：[1]National Engineering Laboratory for Modern Silk,College of Textile and Clothing Engineering,Soochow University

出　　处：《Journal of Wuhan University of Technology(Materials Science)》2011年第6期1044-1048,共5页武汉理工大学学报（材料科学英文版）

基　　金：Funded by the National Natural Science Foundation of China ( Nos. 51173125 and 51075422);the Natural Science Foundation of Jiangsu Province of China (Nos. BK2009147 and BK2010253);the Development Foundation of Suzhou of China (No.SYG201001);the Priority Academic Program Development of Jiangsu Higher Education Institutions [PAPD]

摘　　要：The study aimed to investigate the adhesion, morphology and proliferation of Sprague Dawley （SD） albino rat bone marrow-derived mesenchymal stem cells （BMSCs） by inverted microscope, cell counting, MTT test and laser scanning confocal microscop （LSCM）. On the regenerated A yamamai SF film or blend films, the cell morphology was almost the same as that on collagen （collagen type I） film, the cell adhesion rate was higher than that on plastic cell plate and B mori SF film after 1 h （p 〈 0.01） of culture, and the cell proliferation was significantly higher than that on collagen film （p 〈 0.01） and plastic cell plate （p 〈 0.01）, and also obviously better than that on B mori SF film. On the other hand, the viability of BMSCs in the regenerated A yamamai SF porous scaffold was better than that in B mori SF porous scaffold and medical grade polyvinyl alcohol （PVA） sponge.The study aimed to investigate the adhesion, morphology and proliferation of Sprague Dawley （SD） albino rat bone marrow-derived mesenchymal stem cells （BMSCs） by inverted microscope, cell counting, MTT test and laser scanning confocal microscop （LSCM）. On the regenerated A yamamai SF film or blend films, the cell morphology was almost the same as that on collagen （collagen type I） film, the cell adhesion rate was higher than that on plastic cell plate and B mori SF film after 1 h （p 〈 0.01） of culture, and the cell proliferation was significantly higher than that on collagen film （p 〈 0.01） and plastic cell plate （p 〈 0.01）, and also obviously better than that on B mori SF film. On the other hand, the viability of BMSCs in the regenerated A yamamai SF porous scaffold was better than that in B mori SF porous scaffold and medical grade polyvinyl alcohol （PVA） sponge.

关 键 词：A yamamai SF BMSC ADHESION PROLIFERATION cell probe LSCM 

分 类 号：R318.08[医药卫生—生物医学工程]