Effects of β-TCP Ceramics on Intracellular Ca^(2+) Concentration,Mineralization of Osteoblast and Protein Structure  

作　　者：齐志涛 张启焕 

机构地区：[1]School of Chemical and Biological Engineering,Yancheng Institute of Technology [2]The Bio-material Research and Engineering Center,Wuhan University of Technology

出　　处：《Journal of Wuhan University of Technology(Materials Science)》2011年第6期1064-1067,共4页武汉理工大学学报（材料科学英文版）

基　　金：Funded by the Research Fund of Key Laboratory for Advanced Technology in Environmental Protection of Jiangsu Province (AE201037);the Foundation for Talent Recruitment of Yancheng Institute of Technology (XKR2011007);"973" Chinese National Key Fundamental Research and Development Program (No.G1999064701)

摘　　要：β-TCP, as one of calcium phosphates ceramics, exerts perfect biocompatibility and osteoconductivity, and is clinically used as a bone graft substitute for decades. Consequently, the effects of β-TCP ceramics on intracellular Ca2＋ concentration, mineralization of osteoblast and BSA protein structure were studied. Results showed that β-TCP could increase the intracelluar Ca2＋ concentration and mineralization of osteoblast, indicating that β-TCP ceramics could take part in the organic metabolism and the degradation product had no detrimental effect on osteoblast in vitro. Furthermore, β-TCP ceramics could increase the content of α-helix and β-pleated sheet and change BSA into more ordering structure, those changes might be favorable for the biomineralization after β-TCP ceramics implanted.β-TCP, as one of calcium phosphates ceramics, exerts perfect biocompatibility and osteoconductivity, and is clinically used as a bone graft substitute for decades. Consequently, the effects of β-TCP ceramics on intracellular Ca2＋ concentration, mineralization of osteoblast and BSA protein structure were studied. Results showed that β-TCP could increase the intracelluar Ca2＋ concentration and mineralization of osteoblast, indicating that β-TCP ceramics could take part in the organic metabolism and the degradation product had no detrimental effect on osteoblast in vitro. Furthermore, β-TCP ceramics could increase the content of α-helix and β-pleated sheet and change BSA into more ordering structure, those changes might be favorable for the biomineralization after β-TCP ceramics implanted.

关 键 词：β-TCP ceramics MINERALIZATION OSTEOBLAST [Ca2＋]i protein structure 

分 类 号：TQ174.759[化学工程—陶瓷工业]