人肾癌细胞核因子-κB p65表达及其与微血管生成的关系  

Relationship of nuclear factor-κB p65 expression and angiogenesis in renal cell carcinoma

作　　者：唐爱国[1] 翟水龙[2] 马克钧[1] 贡冰峰[1] 孙浩[1] 

机构地区：[1]江苏大学附属医院泌尿外科,镇江市212001 [2]山西临汾市人民医院

出　　处：《江苏医药》2011年第23期2775-2777,I0001,共4页Jiangsu Medical Journal

基　　金：镇江市社会发展基金项目(SH2010032)

摘　　要：目的探讨人肾癌细胞核因子-κB p65(NF-κB p65)表达与微血管生成的关系。方法免疫组化染色法检测40例肾癌组手术切除的肾癌细胞NF-κB p65表达,通过标记CD34阳性表达检测微血管密度(MVD)。对不同分期肾癌细胞内NF-κB p65阳性表达率及MVD进行比较并分析其与患者生存期关系。对照组10例肾组织取自远离肾癌的正常肾组织。结果 40例肾癌组织中,26例肾癌细胞内NF-κB p65阳性表达,对照组肾组织中未见阳性表达(P<0.01)。其中,晚期肾癌细胞内NF-κB p65阳性表达率明显高于肾癌早期(P<0.05)。与肾癌细胞内NF-κB p65阴性表达者比较,肾癌细胞NF-κB p65阳性表达者MVD数明显升高(P<0.01),而患者5年生存率明显降低(P<0.05)。结论肾癌细胞内NF-κB p65阳性表达与微血管生成密切相关,影响患者病情发展及预后。Objective To investigate the relationship of nuclear factor-κ B p65（NF-κB p65） expression and microvascular angiogenesis in renal cell carcinoma（RCC）.Methods The expression of NF-κB p65 and the microvessel density（MVD） were detected by immunohistochemical staining method in the RCC samples from 40 cases after surgery（group R）.The relationship of the positive expression of NF-κB p65 and MVD was analyzed in different stages of RCC.The results were compared to 10 normal renal samples taken far from RCC（group C）.Results The positive expression rate of NF-κB p65 in group R was 65%（26/40）,which was higher in the cases with late stage of RCC than that in those in the early stage.There was no positive expression of NF-κB p65 in group C.In RCC patients with NF-κB p65 positive expression,the MVD was obviously higher and the 5-year survival rate obviously lower.Conclusion The positive expression of NF-κB p65 is closely associated with microvascular angiogenesis in RCC,which affects the development and progression of RCC.

关 键 词：肾癌 核因子-ΚB P65 微血管密度 

分 类 号：R737[医药卫生—肿瘤]