低强度超声理疗防治犬下颌骨放射性骨坏死  

Prevention of osteoradionecrosis of the mandibule in canine with low intensity ultrasound

作　　者：吴高义[1] 陈磊[2] 朱国雄[1] 刘宝林 王彦亮 

机构地区：[1]济南军区总医院口腔科,250031 [2]济南市口腔医院正畸科 [3]第四军医大学口腔医院颌面外科

出　　处：《中华创伤杂志》2011年第12期1132-1135,共4页Chinese Journal of Trauma

基　　金：第48批中国博士后科学基金资助项目（2010048152）;陕西省科学技术研究发展计划资助项目（2009K17-01J）;山东省优秀中青年科学家科研奖励基金资助项目（BS2011YY067）

摘　　要：目的探讨低强度超声对犬下颌骨骨组织放射线损伤的修复作用，为临床防治颌骨放射性坏死提供新思路。方法建立下颌骨放射线损伤的动物模型，并用低强度超声对其进行治疗，通过微血管密度检测、Micro—CT、丽春红三色染色等观察低强度超声治疗组（实验Ⅱ组）与未用低强度超声治疗组（实验Ⅰ组），并与对照组进行比较。结果成功建立放射线损伤的动物模型。实验Ⅱ组微血管密度大于实验Ⅰ组。实验Ⅱ组骨小梁的骨体积分数、骨小梁厚度、骨表面积和骨体积的比值和骨小梁数量大于实验Ⅰ组（P〈0．05）。丽春红三色染色可见实验Ⅰ组骨细胞大部分消失，松质骨小梁萎缩；实验Ⅱ组见大量骨质形成，形成越来越多的骨小梁，骨小梁边沿可见大量成骨细胞。结论低强度超声对放射线照射后的骨组织具有较好的修复作用。Objective To investigate the repair effect of low intensity ultrasound on radioactive mandibule bone to provide new idea for prevention of osteoradionecrosis of the mandibule after irradiation. Methods The animal models of radioactive mandibular injury were made and treated with low intensity ultrasound. Low intensity ultrasound treatment group （Group Ⅱ ） and not with low intensity ultrasound treatment group （Group I ） were observed by microvessel density detection, micro-CT, ponceau tri- chrome stain and so on, and compared with control group. Then, the obtained data were statistically analyzed. Results The animal models of radioactive mandibular injury were successfully established. The microvessel density in Group Ⅱ was greater than that in Group I . The bone volume fraction, trabecular thickness, bone surface/bone volume and the trabecular number in Group Ⅱ were significantly larger than those in Group I （P 〈 0.05）. The ponceau trichrome staining showed that the osteocytes largely disappeared and that the cancellous bone trabecular atrophied in Group I , while new bone formation, trabecular and numerous osteoblasts around trabecular were largely observed in Group Ⅱ. Conclusion Low intensity ultrasound has a good recovery effect on bone tissue after irradiation.

关 键 词：下颌骨 骨坏死 放射性 超声疗法 微血管密度 

分 类 号：R782[医药卫生—口腔医学]