低成本绿色循环工艺发酵生产丙酸联产维生素B_(12)  

Green and inexpensive circulation process for propionic acid and vitamin B_(12) production by Propionibacterium freudenreichii CCTCC M207015

作　　者：陈飞[1] 张力 冯小海[1] 吴波[1] 徐虹[1] 

机构地区：[1]南京工业大学食品与轻工学院材料化学工程国家重点实验室,南京210009 [2]北京三鸣中食科技发展有限公司,北京100027

出　　处：《生物加工过程》2011年第6期15-20,共6页Chinese Journal of Bioprocess Engineering

基　　金：国家重点基础研究发展计划(973计划)资助项目(2007CB714304);教育部高等学校博士学科点专项科研基金资助项目(20103221120007)

摘　　要：对提取维生素B12后的费氏丙酸杆菌废菌体进行水解处理,考察以菌体水解液作为N源用于丙酸发酵的可行性。利用正交设计得到了提取维生素B12后的废菌体水解优化条件。基于此,构建利用植物纤维床反应器固定化生产丙酸联产维生素B12的低成本绿色循环工艺。结果表明:在4.5 L的发酵体系中,单批次总糖质量浓度为200 g/L,发酵进行了5批次共1 192 h,丙酸生成总量为2 328.75 g,单批次丙酸质量浓度103.50 g/L,丙酸生产效率达0.43 g/(L.h),干菌质量浓度达到19.52 g/L。将菌体注入微好氧发酵罐中发酵获得112.8 mg/L维生素B12。Waste propionibacterium cells recovered from vitamin B12 extraction were used as nitrogen source for propionic acid fermentation.The optimizing conditions of bacteria hydrolysis were determined by the orthogonal design.Based on the above results,an immobilized fermentation unit with plant fibrous-bed bioreactor（PFB） combined with membrane separation was constructed and applied in the joint production of propionic acid and vitamin B12.By using hydrolyzed waste cells as alternative nitrogen source,propionic acid and vitamin B12 were produced with the green and inexpensive circulation process.The results showed that propionic acid yield reached a total of 2 328.75 g with a working volume of 4.5 L after five batches（1 192 h） in PFB immobilized coupling fermentation（single-batch glucose concentration was 200 g/L）,single-batch propionic acid concentration was reached 103.50 g/L with productivity of 0.43 g/（L·h）.With the separation combined with fermentation,cell concentration gradually increased and cell dry weight reached 19.52 g/L.The whole cells were washed into the micro-aerobic bioreactor to synthesis 112.8 mg/L vitamin B12.

关 键 词：植物纤维床反应器 费氏丙酸杆菌 丙酸 维生素B12 废菌体 

分 类 号：TQ921[轻工技术与工程—发酵工程] TQ924